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- W2023498857 abstract "Abstract Two methods were compared for the detection of solubilized human placental 3β-hydroxysteroid dehydrogenase activity in polyacrylamide gels following electrophoresis: (i) nitroblue tetrazolium-nicotinamide adenine dinucleotide (NBT-NAD) coupled histochemical incubations of entire gels and (2) direct radiochemical assay of 1.5-mm gel slices. Both procedures utilized pregnenolone as enzyme substrate. The histochemical method showed “nothing dehydrogenase” activity in that gels developed identical banding patterns in the presence and absence of substrate. The method also consistently failed to indicate enzyme activity at loci easily determined by radiochemical assay." @default.
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- W2023498857 date "1977-03-01" @default.
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- W2023498857 title "Localizing steroid dehydrogenase activity on acrylamide gels: The perils of “histochemistry”" @default.
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- W2023498857 doi "https://doi.org/10.1016/0003-2697(77)90025-2" @default.
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