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- W2023503013 abstract "DNA topoisomerases are enzymes that control DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through the transient gaps. Consequently, these enzymes play a crucial role in the regulation of the physiological function of the genome. Beyond their normal functions, topoisomerases are important cellular targets in the treatment of human cancers. In this review we summarize current protocols for extracting and purifying DNA topoisomerases, and for separating subtypes and isoforms of these enzymes. Furthermore, we discuss methods for measuring the catalytic activity of topoisomerases and for monitoring the molecular effects of topoisomerase-directed antitumor drugs in cell-free assays." @default.
- W2023503013 created "2016-06-24" @default.
- W2023503013 creator A5058562579 @default.
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- W2023503013 date "1996-09-01" @default.
- W2023503013 modified "2023-10-10" @default.
- W2023503013 title "Separation and functional analysis of eukaryotic DNA topoisomerases by chromatography and electrophoresis" @default.
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- W2023503013 doi "https://doi.org/10.1016/0378-4347(96)00152-1" @default.
- W2023503013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8906479" @default.
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