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- W2023505746 abstract "Summary A quantitative method is described for the chemical assay of progesterone and Δ 4 -pregnene-20β-ol-3-one. The tissues were extracted with ethyl acetate, followed by vacuum distillation and partitioning of the residue between Skellysolve B - 70% methanol and benzene. The progestins were separated and further purified by paper chromatography using the Bush Skellysolve B - 95% methanol system, eluted, and rechromatographed in the same system. Progesterone-4-C 14 (0.08µg; 3,500 c.p.m.) was added at the time of initial extraction to estimate recovery of progesterone during each assay. The radiochemical purity of the compound was 93–94%. About 4% of the radioactivity remained in the tissue during initial extraction and approximately 16% was lost during each elution of the paper chromatograms. Average recovery of progesterone-4-C 14 was 40% for 224 samples of cow tissues (standard error 0.7%) and 51% for 114 samples of sow tissues (standard error 1.1%). No Δ 4 -pregnene-20β-ol-3-one was detected in sow tissues." @default.
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- W2023505746 date "1961-02-01" @default.
- W2023505746 modified "2023-09-29" @default.
- W2023505746 title "Quantitative Methods for Determining Progestins in Corpora Lutea, Ovaries, and Adrenals of the Cow and Sow Using Progesterone-4-C14 as a Measure of Recovery of Extracted Hormone" @default.
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- W2023505746 doi "https://doi.org/10.3168/jds.s0022-0302(61)89735-x" @default.
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