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• W2023509216 endingPage "e13979" @default.
• W2023509216 startingPage "e13979" @default.
• W2023509216 abstract "Epstein-Barr virus (EBV) is able to drive the transformation of B-cells, resulting in the generation of lymphoblastoid cell lines (LCLs) in vitro. EBV nuclear proteins EBNA3A and EBNA3C are necessary for efficient transformation, while EBNA3B is dispensable. We describe a transcriptome analysis of BL31 cells infected with a series of EBNA3-knockout EBVs, including one deleted for all three EBNA3 genes. Using Affymetrix Exon 1.0 ST microarrays analysed with the MMBGX algorithm, we have identified over 1000 genes whose regulation by EBV requires one of the EBNA3s. Remarkably, a third of the genes identified require more than one EBNA3 for their regulation, predominantly EBNA3C co-operating with either EBNA3B, EBNA3A or both. The microarray was validated by real-time PCR, while ChIP analysis of a selection of co-operatively repressed promoters indicates a role for polycomb group complexes. Targets include genes involved in apoptosis, cell migration and B-cell differentiation, and show a highly significant but subtle alteration in genes involved in mitosis. In order to assess the relevance of the BL31 system to LCLs, we analysed the transcriptome of a set of EBNA3B knockout (3BKO) LCLs. Around a third of the genes whose expression level in LCLs was altered in the absence of EBNA3B were also altered in 3BKO-BL31 cell lines. Among these are TERT and TCL1A, implying that EBV-induced changes in the expression of these genes are not required for B-cell transformation. We also identify 26 genes that require both EBNA3A and EBNA3B for their regulation in LCLs. Together, this shows the complexity of the interaction between EBV and its host, whereby multiple EBNA3 proteins co-operate to modulate the behaviour of the host cell." @default.
• W2023509216 created "2016-06-24" @default.
• W2023509216 creator A5010947173 @default.
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• W2023509216 creator A5058585899 @default.
• W2023509216 creator A5065576952 @default.
• W2023509216 creator A5067497578 @default.
• W2023509216 date "2010-11-15" @default.
• W2023509216 modified "2023-10-10" @default.
• W2023509216 title "Extensive Co-Operation between the Epstein-Barr Virus EBNA3 Proteins in the Manipulation of Host Gene Expression and Epigenetic Chromatin Modification" @default.
• W2023509216 cites W1253046850 @default.
• W2023509216 cites W1486000535 @default.
• W2023509216 cites W1488614375 @default.
• W2023509216 cites W1514074978 @default.
• W2023509216 cites W1524551107 @default.
• W2023509216 cites W1547608553 @default.
• W2023509216 cites W1562827330 @default.
• W2023509216 cites W1578954095 @default.
• W2023509216 cites W1591897457 @default.
• W2023509216 cites W1606634120 @default.
• W2023509216 cites W1963699983 @default.
• W2023509216 cites W1966082666 @default.
• W2023509216 cites W1968931043 @default.
• W2023509216 cites W1969322596 @default.
• W2023509216 cites W1973279069 @default.
• W2023509216 cites W1973709348 @default.
• W2023509216 cites W1975562645 @default.
• W2023509216 cites W1978032326 @default.
• W2023509216 cites W1979399955 @default.
• W2023509216 cites W1979401674 @default.
• W2023509216 cites W1986628066 @default.
• W2023509216 cites W1988979049 @default.
• W2023509216 cites W1991253015 @default.
• W2023509216 cites W1992642201 @default.
• W2023509216 cites W1992921284 @default.
• W2023509216 cites W2005862926 @default.
• W2023509216 cites W2008254200 @default.
• W2023509216 cites W2008693647 @default.
• W2023509216 cites W2018443217 @default.
• W2023509216 cites W2028716241 @default.
• W2023509216 cites W2036024016 @default.
• W2023509216 cites W2038340280 @default.
• W2023509216 cites W2050899725 @default.
• W2023509216 cites W2051301388 @default.
• W2023509216 cites W2060273300 @default.
• W2023509216 cites W2061541027 @default.
• W2023509216 cites W2062729499 @default.
• W2023509216 cites W2064945432 @default.
• W2023509216 cites W2068549702 @default.
• W2023509216 cites W2071775939 @default.
• W2023509216 cites W2073989067 @default.
• W2023509216 cites W2085161568 @default.
• W2023509216 cites W2085220509 @default.
• W2023509216 cites W2088422296 @default.
• W2023509216 cites W2090402866 @default.
• W2023509216 cites W2090619917 @default.
• W2023509216 cites W2095024877 @default.
• W2023509216 cites W2095587862 @default.
• W2023509216 cites W2096801391 @default.
• W2023509216 cites W2101139969 @default.
• W2023509216 cites W2105025692 @default.
• W2023509216 cites W2112757981 @default.
• W2023509216 cites W2112821935 @default.
• W2023509216 cites W2114834254 @default.
• W2023509216 cites W2116489876 @default.
• W2023509216 cites W2120060323 @default.
• W2023509216 cites W2120996479 @default.
• W2023509216 cites W2124987874 @default.
• W2023509216 cites W2138593912 @default.
• W2023509216 cites W2139700920 @default.
• W2023509216 cites W2141053032 @default.
• W2023509216 cites W2141616119 @default.
• W2023509216 cites W2146198196 @default.
• W2023509216 cites W2147641075 @default.
• W2023509216 cites W2148343879 @default.
• W2023509216 cites W2149766132 @default.
• W2023509216 cites W2149851884 @default.
• W2023509216 cites W2152523857 @default.
• W2023509216 cites W2153139469 @default.
• W2023509216 cites W2157184487 @default.
• W2023509216 cites W2159452860 @default.
• W2023509216 cites W2161663883 @default.
• W2023509216 cites W2162372318 @default.
• W2023509216 cites W2169694216 @default.
• W2023509216 cites W2170036207 @default.
• W2023509216 cites W2170550731 @default.
• W2023509216 cites W2170555802 @default.
• W2023509216 cites W2172230584 @default.
• W2023509216 cites W2188700200 @default.
• W2023509216 cites W8133033 @default.
• W2023509216 doi "https://doi.org/10.1371/journal.pone.0013979" @default.
• W2023509216 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2981562" @default.
• W2023509216 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21085583" @default.
• W2023509216 hasPublicationYear "2010" @default.
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