Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023522077> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2023522077 endingPage "214" @default.
- W2023522077 startingPage "214" @default.
- W2023522077 abstract "It has been suggested that glucose metabolites and insulin are the most important factors inducing ATP-citrate lyase (ACL) by a high carbohydrate diet. We have used a primary culture of rat hepatocytes to confirm the role of glucose and insulin in terms of ACL gene expression. The results showed that glucose displayed a direct effect on ACL gene expression and the insulin helps the glucose effect. The nucleotide sequences from -512 to -485 of the ACL promoter are highly homologous (70%) to the sequences surrounding the carbohydrate response element (ChoRE) of the S14 gene. The gel retardation analysis using ChoRE of the S14 gene showed that the ACL promoter which contains the ChoRE-like sequence specifically inhibited the formation of the complex by the nuclear proteins isolated from rat liver. To localize the regions which are involved in the regulation of ACL gene expression, transient expression assay using ACL promoter-CAT (chloramphenicol acetyltransferase) constructs containing various lengths of a 5' flanking region of the ACL gene were carried out. The proximal promoter region -419 to -1 containing several potential Sp1 binding sites showed the strong enhancing effect, which increases the transcription of CAT genes in the various cell lines, such as the CHO (Chinese hamster ovary) cell, the HepG2 cell, and primary cultured rat hepatocytes. In response to glucose, among the ACL promoter-CAT constructs, only pNP33-CAT (-1342 to -1) showed a 2.64 fold increase in CAT activity by a high concentration of glucose. The activation of ACL gene expression by glucose seems to be regulated in a complicated manner involving interactions between the contexts of the several sequence elements and various transacting factors, which is not a simple mechanism directed only by a short sequence element." @default.
- W2023522077 created "2016-06-24" @default.
- W2023522077 creator A5019257755 @default.
- W2023522077 creator A5019900739 @default.
- W2023522077 creator A5043920801 @default.
- W2023522077 creator A5070792652 @default.
- W2023522077 creator A5083550838 @default.
- W2023522077 date "1996-01-01" @default.
- W2023522077 modified "2023-10-18" @default.
- W2023522077 title "Regulation of ATP-citrate lyase gene transcription" @default.
- W2023522077 doi "https://doi.org/10.3349/ymj.1996.37.3.214" @default.
- W2023522077 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8826788" @default.
- W2023522077 hasPublicationYear "1996" @default.
- W2023522077 type Work @default.
- W2023522077 sameAs 2023522077 @default.
- W2023522077 citedByCount "10" @default.
- W2023522077 countsByYear W20235220772012 @default.
- W2023522077 countsByYear W20235220772014 @default.
- W2023522077 countsByYear W20235220772020 @default.
- W2023522077 crossrefType "journal-article" @default.
- W2023522077 hasAuthorship W2023522077A5019257755 @default.
- W2023522077 hasAuthorship W2023522077A5019900739 @default.
- W2023522077 hasAuthorship W2023522077A5043920801 @default.
- W2023522077 hasAuthorship W2023522077A5070792652 @default.
- W2023522077 hasAuthorship W2023522077A5083550838 @default.
- W2023522077 hasBestOaLocation W20235220772 @default.
- W2023522077 hasConcept C101762097 @default.
- W2023522077 hasConcept C104317684 @default.
- W2023522077 hasConcept C111133373 @default.
- W2023522077 hasConcept C132376346 @default.
- W2023522077 hasConcept C138885662 @default.
- W2023522077 hasConcept C150194340 @default.
- W2023522077 hasConcept C153911025 @default.
- W2023522077 hasConcept C165864922 @default.
- W2023522077 hasConcept C175656101 @default.
- W2023522077 hasConcept C179926584 @default.
- W2023522077 hasConcept C181199279 @default.
- W2023522077 hasConcept C204999636 @default.
- W2023522077 hasConcept C2780730277 @default.
- W2023522077 hasConcept C41895202 @default.
- W2023522077 hasConcept C54355233 @default.
- W2023522077 hasConcept C55493867 @default.
- W2023522077 hasConcept C81885089 @default.
- W2023522077 hasConcept C86803240 @default.
- W2023522077 hasConceptScore W2023522077C101762097 @default.
- W2023522077 hasConceptScore W2023522077C104317684 @default.
- W2023522077 hasConceptScore W2023522077C111133373 @default.
- W2023522077 hasConceptScore W2023522077C132376346 @default.
- W2023522077 hasConceptScore W2023522077C138885662 @default.
- W2023522077 hasConceptScore W2023522077C150194340 @default.
- W2023522077 hasConceptScore W2023522077C153911025 @default.
- W2023522077 hasConceptScore W2023522077C165864922 @default.
- W2023522077 hasConceptScore W2023522077C175656101 @default.
- W2023522077 hasConceptScore W2023522077C179926584 @default.
- W2023522077 hasConceptScore W2023522077C181199279 @default.
- W2023522077 hasConceptScore W2023522077C204999636 @default.
- W2023522077 hasConceptScore W2023522077C2780730277 @default.
- W2023522077 hasConceptScore W2023522077C41895202 @default.
- W2023522077 hasConceptScore W2023522077C54355233 @default.
- W2023522077 hasConceptScore W2023522077C55493867 @default.
- W2023522077 hasConceptScore W2023522077C81885089 @default.
- W2023522077 hasConceptScore W2023522077C86803240 @default.
- W2023522077 hasIssue "3" @default.
- W2023522077 hasLocation W20235220771 @default.
- W2023522077 hasLocation W20235220772 @default.
- W2023522077 hasLocation W20235220773 @default.
- W2023522077 hasOpenAccess W2023522077 @default.
- W2023522077 hasPrimaryLocation W20235220771 @default.
- W2023522077 hasRelatedWork W1570674964 @default.
- W2023522077 hasRelatedWork W1981892740 @default.
- W2023522077 hasRelatedWork W2023522077 @default.
- W2023522077 hasRelatedWork W2028666390 @default.
- W2023522077 hasRelatedWork W2057875675 @default.
- W2023522077 hasRelatedWork W2060131368 @default.
- W2023522077 hasRelatedWork W2111182449 @default.
- W2023522077 hasRelatedWork W2156042726 @default.
- W2023522077 hasRelatedWork W2172226517 @default.
- W2023522077 hasRelatedWork W2409015522 @default.
- W2023522077 hasVolume "37" @default.
- W2023522077 isParatext "false" @default.
- W2023522077 isRetracted "false" @default.
- W2023522077 magId "2023522077" @default.
- W2023522077 workType "article" @default.