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• W2023530134 abstract "Microbiology and ImmunologyVolume 42, Issue 3 p. 143-150 MinireviewFree Access Production of a Diverse Repertoire of Human Antibodies in Genetically Engineered Mice Isao Ishida, Corresponding Author Isao Ishida Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Yokohama, Kanagawa, 236-0004 JapanAddress correspondence to Dr. Isao Ishida, Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan. E-mail: i-ishida@kirin.co.jpSearch for more papers by this authorHitoshi Yoshida, Hitoshi Yoshida Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Yokohama, Kanagawa, 236-0004 JapanSearch for more papers by this authorKazuma Tomizuka, Kazuma Tomizuka Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Yokohama, Kanagawa, 236-0004 JapanSearch for more papers by this author Isao Ishida, Corresponding Author Isao Ishida Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Yokohama, Kanagawa, 236-0004 JapanAddress correspondence to Dr. Isao Ishida, Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan. E-mail: i-ishida@kirin.co.jpSearch for more papers by this authorHitoshi Yoshida, Hitoshi Yoshida Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Yokohama, Kanagawa, 236-0004 JapanSearch for more papers by this authorKazuma Tomizuka, Kazuma Tomizuka Central Laboratories for Key Technology, Kirin Brewery Co., Ltd., Yokohama, Kanagawa, 236-0004 JapanSearch for more papers by this author First published: 14 November 2013 https://doi.org/10.1111/j.1348-0421.1998.tb02264.xCitations: 8AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abbreviations BAC bacterial artificial chromosome C constant CDR complementarity determining region D diversity ELISA enzyme-linked immunosorbent assay ES cell embryonic stem cell hChr. human chromosome hIgH human Ig heavy chain hIgL human Ig light chain HSA human serum albumin Ig immunoglobulin J joining Mb megabase MH(ES)cell microcell-hybrid embryonic stem cell MMCT microcell-mediated chromosome transfer PCR polymerase chain reaction V variable YAC yeast artificial chromosome. References 1Bruggemann, M., Caskey, H.M., Teale, C., Waldmann, H., Williams, G.T., Surani, M.A., and Neuberger, M.S. 1989. A repertoire of monoclonal antibodies with human heavy chains from transgenic mice. Proc. Natl. Acad. Sci. U.S.A. 86: 6709– 6713. CrossrefCASPubMedWeb of Science®Google Scholar 2Buttin, G. 1987. Exogenous Ig gene rearrangement in transgenic mice: a new strategy for human monoclonal antibody production? 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