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• W2023532050 abstract "Objective: To find out whether measurements of intracapillary haemoglobin oxygenation made with the non-invasive Erlangen microlightguide spectrophotometer (EMPHO II) provided accurate data about the oxygen supply as well as the blood flow to free flaps in rats and humans. Design: Experimental, and prospective open clinical study. Setting: University hospital, Germany. Animals and subjects: Thirty male Wistar rats and 20 patients who underwent microsurgical transplant of free flaps (radial forearm, n = 8; osteocutaneous fibula, n = 7; and myocutaneous from the lateral thigh, n = 5). Interventions: Measurement of the haemoglobin oxygenation of the skin before, during, and after transfer of the flap. Main outcome measures: Reproducibility and mean curves for haemoglobin oxygenation and haemoglobin concentration. Results: All values were reproducible, and there were only slight fluctuations. Uninjured skin in rats (baseline value) oxygenation of the total haemoglobin concentration ranged from 15% to 45% (mean 23%). After the flaps were raised there was a slight increase (to a mean of 37%), probably as a result of reactive hyperaemia. There was a rapid decrease within a few minutes of arterial occlusion, and residual oxygenation of up to 20% after perfusion stopped. The flap was totally deoxygenated after 1 h. Venous occlusion caused a similar pattern and all flaps were deoxygenated by 30 min. In the clinical study ligation of the vascular pedicle caused a massive reduction in values but after anastomosis there was significantly higher haemoglobin oxygenation (P=0.05) and this continued to increase postoperatively. There were no complications and oxygenation gradually and continuously decreased from the base of the flap to the periphery. Conclusion: The non-invasive EMPHO II provides reliable and easily assessable data about the circulation and supply of oxygen to a transplanted free flap." @default.
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• W2023532050 date "1996-12-01" @default.
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• W2023532050 title "Monitoring of flaps by measurement of intracapillary haemoglobin oxygenation with EMPHO 11: experimental and clinical study" @default.
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• W2023532050 doi "https://doi.org/10.1016/s0266-4356(96)90250-8" @default.
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