Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023548836> ?p ?o ?g. }
- W2023548836 endingPage "733" @default.
- W2023548836 startingPage "729" @default.
- W2023548836 abstract "The binding and uptake of plasmid DNA encoding luciferase reporter gene (pCMV-Luc) were studied in vitro using cultured mouse peritoneal macrophages. A significant and time-dependent cellular association of [32P]pCMV-Luc with resident macrophages was observed at 37 °C and this decreased at 4 °C. The binding at 4 °C was saturable and a Scatchard plot gave a maximum binding capacity of 0.81 μg/mg-protein and a dissociation constant of 0.30 μg/ml. The binding of [32P]pCMV-Luc was inhibited by polyinosinic acid, dextran sulfate and salmon sperm DNA, but not by polycytidylic acid, dextran and EDTA. A confocal microscopic study demonstrated that fluorescein-labeled pCMV-Luc was internalized at 37 °C while only cell surface binding occurred at 4 °C. No significant luciferase gene expression was obtained after incubation with a high concentration (100 μg/ml) of pCMV-Luc. These data suggest that plasmid DNA is taken up by macrophages via a mechanism mediated by a receptor like the macrophage scavenger receptor." @default.
- W2023548836 created "2016-06-24" @default.
- W2023548836 creator A5020049683 @default.
- W2023548836 creator A5035791716 @default.
- W2023548836 creator A5049821646 @default.
- W2023548836 creator A5051052380 @default.
- W2023548836 creator A5059952072 @default.
- W2023548836 creator A5082694156 @default.
- W2023548836 date "1998-04-01" @default.
- W2023548836 modified "2023-10-03" @default.
- W2023548836 title "Involvement of Specific Mechanism in Plasmid DNA Uptake by Mouse Peritoneal Macrophages" @default.
- W2023548836 cites W1510227107 @default.
- W2023548836 cites W1526755525 @default.
- W2023548836 cites W1570307043 @default.
- W2023548836 cites W1636901091 @default.
- W2023548836 cites W165015491 @default.
- W2023548836 cites W1972489922 @default.
- W2023548836 cites W1979956993 @default.
- W2023548836 cites W1982954591 @default.
- W2023548836 cites W1989744185 @default.
- W2023548836 cites W1994916925 @default.
- W2023548836 cites W2006258756 @default.
- W2023548836 cites W2010701664 @default.
- W2023548836 cites W2038484123 @default.
- W2023548836 cites W2042863201 @default.
- W2023548836 cites W2050181420 @default.
- W2023548836 cites W2051745497 @default.
- W2023548836 cites W2062860313 @default.
- W2023548836 cites W2064244952 @default.
- W2023548836 cites W2068505853 @default.
- W2023548836 cites W2069533751 @default.
- W2023548836 cites W2073410991 @default.
- W2023548836 cites W2087797124 @default.
- W2023548836 cites W2090835365 @default.
- W2023548836 cites W2092246905 @default.
- W2023548836 cites W2116386276 @default.
- W2023548836 cites W2118877270 @default.
- W2023548836 cites W2129013065 @default.
- W2023548836 cites W2149960019 @default.
- W2023548836 cites W2159264850 @default.
- W2023548836 doi "https://doi.org/10.1006/bbrc.1998.8521" @default.
- W2023548836 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9588183" @default.
- W2023548836 hasPublicationYear "1998" @default.
- W2023548836 type Work @default.
- W2023548836 sameAs 2023548836 @default.
- W2023548836 citedByCount "69" @default.
- W2023548836 countsByYear W20235488362012 @default.
- W2023548836 countsByYear W20235488362014 @default.
- W2023548836 countsByYear W20235488362015 @default.
- W2023548836 countsByYear W20235488362017 @default.
- W2023548836 countsByYear W20235488362018 @default.
- W2023548836 crossrefType "journal-article" @default.
- W2023548836 hasAuthorship W2023548836A5020049683 @default.
- W2023548836 hasAuthorship W2023548836A5035791716 @default.
- W2023548836 hasAuthorship W2023548836A5049821646 @default.
- W2023548836 hasAuthorship W2023548836A5051052380 @default.
- W2023548836 hasAuthorship W2023548836A5059952072 @default.
- W2023548836 hasAuthorship W2023548836A5082694156 @default.
- W2023548836 hasConcept C104317684 @default.
- W2023548836 hasConcept C105702510 @default.
- W2023548836 hasConcept C111335760 @default.
- W2023548836 hasConcept C121332964 @default.
- W2023548836 hasConcept C153911025 @default.
- W2023548836 hasConcept C170493617 @default.
- W2023548836 hasConcept C185592680 @default.
- W2023548836 hasConcept C202751555 @default.
- W2023548836 hasConcept C22744801 @default.
- W2023548836 hasConcept C2778015030 @default.
- W2023548836 hasConcept C2778536324 @default.
- W2023548836 hasConcept C54009773 @default.
- W2023548836 hasConcept C552990157 @default.
- W2023548836 hasConcept C55493867 @default.
- W2023548836 hasConcept C62520636 @default.
- W2023548836 hasConcept C86803240 @default.
- W2023548836 hasConcept C91881484 @default.
- W2023548836 hasConceptScore W2023548836C104317684 @default.
- W2023548836 hasConceptScore W2023548836C105702510 @default.
- W2023548836 hasConceptScore W2023548836C111335760 @default.
- W2023548836 hasConceptScore W2023548836C121332964 @default.
- W2023548836 hasConceptScore W2023548836C153911025 @default.
- W2023548836 hasConceptScore W2023548836C170493617 @default.
- W2023548836 hasConceptScore W2023548836C185592680 @default.
- W2023548836 hasConceptScore W2023548836C202751555 @default.
- W2023548836 hasConceptScore W2023548836C22744801 @default.
- W2023548836 hasConceptScore W2023548836C2778015030 @default.
- W2023548836 hasConceptScore W2023548836C2778536324 @default.
- W2023548836 hasConceptScore W2023548836C54009773 @default.
- W2023548836 hasConceptScore W2023548836C552990157 @default.
- W2023548836 hasConceptScore W2023548836C55493867 @default.
- W2023548836 hasConceptScore W2023548836C62520636 @default.
- W2023548836 hasConceptScore W2023548836C86803240 @default.
- W2023548836 hasConceptScore W2023548836C91881484 @default.
- W2023548836 hasFunder F4320309773 @default.
- W2023548836 hasFunder F4320320912 @default.
- W2023548836 hasIssue "3" @default.
- W2023548836 hasLocation W20235488361 @default.
- W2023548836 hasLocation W20235488362 @default.
- W2023548836 hasOpenAccess W2023548836 @default.