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- W2023557079 abstract "A series of pBR322 recombinants containing the intact early region and origin of replication of polyoma were constructed and tested for their ability to replicate in permissive mouse cells. During the first 60 hours after transfection of these plasmids into mouse cells there was an accumulation of material similar to that observed with non-cloned polyoma DNA, though none of the plasmids replicated up to as high a copy number as non-cloned polyoma DNA. The mouse-replicated plasmid DNAs had undergone changes in their methylation patterns consistent with their having been propagated in eukaryotic cells. They could be recovered efficiently by transfection back into Escherichia coli, and the structure of the recovered plasmids indicated that at least small plasmids were faithfully replicated in mouse cells." @default.
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- W2023557079 date "1981-09-01" @default.
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- W2023557079 title "Replication of polyoma plasmid recombinants in mouse cells" @default.
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- W2023557079 doi "https://doi.org/10.1016/0022-2836(81)90229-1" @default.
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