Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023561032> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2023561032 endingPage "91" @default.
- W2023561032 startingPage "85" @default.
- W2023561032 abstract "A cell-free expression system using an <i>Escherichia coli</i> extract was adapted for large-scale expression and purification of mammalian membrane proteins. The system was tested with a set of human membrane proteins of different sizes, numbers of transmembrane domains, oligomeric arrangements, and native membrane locations. Tens of milligrams of protein were readily expressed and purified from an overnight cell-free reaction. Both reaction ‘mode A’ (proteins were expressed as precipitant) and ‘mode B’ (proteins were expressed in the presence of mild detergents to keep them soluble) were investigated. The combination of ‘mode B’ and the right detergents, used in the subsequent extraction and purification steps, is critical for obtaining properly folded proteins (CX32 and VDAC1) that can be crystallized and diffracted (VDAC1). The <i>E. coli </i>cell-free system is capable of efficient expression of many mammalian membrane proteins. However, fine-tuning of the system, especially to facilitate proper protein folding, will be required for each specific target." @default.
- W2023561032 created "2016-06-24" @default.
- W2023561032 creator A5005264649 @default.
- W2023561032 creator A5078487092 @default.
- W2023561032 creator A5081015831 @default.
- W2023561032 date "2010-01-01" @default.
- W2023561032 modified "2023-10-12" @default.
- W2023561032 title "An <i>Escherichia coli</i>-Based Cell-Free System for Large-Scale Production of Functional Mammalian Membrane Proteins Suitable for X-Ray Crystallography" @default.
- W2023561032 cites W1587481053 @default.
- W2023561032 cites W1963528492 @default.
- W2023561032 cites W1982214572 @default.
- W2023561032 cites W1986199954 @default.
- W2023561032 cites W1994001831 @default.
- W2023561032 cites W2018593981 @default.
- W2023561032 cites W2025892003 @default.
- W2023561032 cites W2060528969 @default.
- W2023561032 cites W2070412459 @default.
- W2023561032 cites W2080803336 @default.
- W2023561032 cites W2085881360 @default.
- W2023561032 cites W2085924667 @default.
- W2023561032 cites W2105852442 @default.
- W2023561032 cites W2121127710 @default.
- W2023561032 cites W2129141040 @default.
- W2023561032 cites W2134087185 @default.
- W2023561032 cites W2134180405 @default.
- W2023561032 cites W2142570996 @default.
- W2023561032 cites W2163733734 @default.
- W2023561032 doi "https://doi.org/10.1159/000283512" @default.
- W2023561032 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2919436" @default.
- W2023561032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20160448" @default.
- W2023561032 hasPublicationYear "2010" @default.
- W2023561032 type Work @default.
- W2023561032 sameAs 2023561032 @default.
- W2023561032 citedByCount "14" @default.
- W2023561032 countsByYear W20235610322012 @default.
- W2023561032 countsByYear W20235610322013 @default.
- W2023561032 countsByYear W20235610322014 @default.
- W2023561032 countsByYear W20235610322015 @default.
- W2023561032 countsByYear W20235610322016 @default.
- W2023561032 countsByYear W20235610322017 @default.
- W2023561032 countsByYear W20235610322018 @default.
- W2023561032 crossrefType "journal-article" @default.
- W2023561032 hasAuthorship W2023561032A5005264649 @default.
- W2023561032 hasAuthorship W2023561032A5078487092 @default.
- W2023561032 hasAuthorship W2023561032A5081015831 @default.
- W2023561032 hasBestOaLocation W20235610321 @default.
- W2023561032 hasConcept C104317684 @default.
- W2023561032 hasConcept C144647389 @default.
- W2023561032 hasConcept C1491633281 @default.
- W2023561032 hasConcept C170493617 @default.
- W2023561032 hasConcept C185592680 @default.
- W2023561032 hasConcept C24530287 @default.
- W2023561032 hasConcept C2778665413 @default.
- W2023561032 hasConcept C3675279 @default.
- W2023561032 hasConcept C41625074 @default.
- W2023561032 hasConcept C547475151 @default.
- W2023561032 hasConcept C55493867 @default.
- W2023561032 hasConcept C86803240 @default.
- W2023561032 hasConcept C95444343 @default.
- W2023561032 hasConceptScore W2023561032C104317684 @default.
- W2023561032 hasConceptScore W2023561032C144647389 @default.
- W2023561032 hasConceptScore W2023561032C1491633281 @default.
- W2023561032 hasConceptScore W2023561032C170493617 @default.
- W2023561032 hasConceptScore W2023561032C185592680 @default.
- W2023561032 hasConceptScore W2023561032C24530287 @default.
- W2023561032 hasConceptScore W2023561032C2778665413 @default.
- W2023561032 hasConceptScore W2023561032C3675279 @default.
- W2023561032 hasConceptScore W2023561032C41625074 @default.
- W2023561032 hasConceptScore W2023561032C547475151 @default.
- W2023561032 hasConceptScore W2023561032C55493867 @default.
- W2023561032 hasConceptScore W2023561032C86803240 @default.
- W2023561032 hasConceptScore W2023561032C95444343 @default.
- W2023561032 hasIssue "2" @default.
- W2023561032 hasLocation W20235610321 @default.
- W2023561032 hasLocation W20235610322 @default.
- W2023561032 hasLocation W20235610323 @default.
- W2023561032 hasLocation W20235610324 @default.
- W2023561032 hasOpenAccess W2023561032 @default.
- W2023561032 hasPrimaryLocation W20235610321 @default.
- W2023561032 hasRelatedWork W1546661123 @default.
- W2023561032 hasRelatedWork W2008963721 @default.
- W2023561032 hasRelatedWork W2343827291 @default.
- W2023561032 hasRelatedWork W2391732167 @default.
- W2023561032 hasRelatedWork W2462958301 @default.
- W2023561032 hasRelatedWork W2487646191 @default.
- W2023561032 hasRelatedWork W2592567520 @default.
- W2023561032 hasRelatedWork W2769332474 @default.
- W2023561032 hasRelatedWork W295885957 @default.
- W2023561032 hasRelatedWork W4377861143 @default.
- W2023561032 hasVolume "18" @default.
- W2023561032 isParatext "false" @default.
- W2023561032 isRetracted "false" @default.
- W2023561032 magId "2023561032" @default.
- W2023561032 workType "article" @default.