Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023562484> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2023562484 endingPage "407" @default.
- W2023562484 startingPage "402" @default.
- W2023562484 abstract "An immunogold postembedding method for the ultrastructural localization of immunoglobulin G (lgG), IgA, IgM, C3, fibrinogen, and kappa and lambda immunoglobulin light chains was established. The method was performed on surplus tissue from renal biopsies fixed in 2% glutaraldehyde and embedded in acrylic LR White resin. Fifteen cases were studied and the localization of antigen in the dense deposits in glomeruli compared with the immunofluorescence (IF) findings on the same cases. The results obtained by IF and immunoelectron microscopy (IEM) were comparable for IgG and IgA. In most instances, IgM and C3 were more intensely labeled by IF than IEM. Some discrepancies were noted for fibrinogen. It is postulated that these differences are related to a partial loss of antigenicity due to fixation or chemical reaction with the embedding medium. The advantage of IEM resides in localizing antigens in very small deposits seen in early stages of glomerulonephritis. The method can supplement IF in diagnostically difficult cases or be substituted for it when material for IF is not available. An immunogold postembedding method for the ultrastructural localization of immunoglobulin G (lgG), IgA, IgM, C3, fibrinogen, and kappa and lambda immunoglobulin light chains was established. The method was performed on surplus tissue from renal biopsies fixed in 2% glutaraldehyde and embedded in acrylic LR White resin. Fifteen cases were studied and the localization of antigen in the dense deposits in glomeruli compared with the immunofluorescence (IF) findings on the same cases. The results obtained by IF and immunoelectron microscopy (IEM) were comparable for IgG and IgA. In most instances, IgM and C3 were more intensely labeled by IF than IEM. Some discrepancies were noted for fibrinogen. It is postulated that these differences are related to a partial loss of antigenicity due to fixation or chemical reaction with the embedding medium. The advantage of IEM resides in localizing antigens in very small deposits seen in early stages of glomerulonephritis. The method can supplement IF in diagnostically difficult cases or be substituted for it when material for IF is not available." @default.
- W2023562484 created "2016-06-24" @default.
- W2023562484 creator A5004769568 @default.
- W2023562484 creator A5021680065 @default.
- W2023562484 creator A5024583991 @default.
- W2023562484 creator A5045047579 @default.
- W2023562484 creator A5049715758 @default.
- W2023562484 creator A5050217682 @default.
- W2023562484 creator A5051656604 @default.
- W2023562484 date "1989-11-01" @default.
- W2023562484 modified "2023-09-23" @default.
- W2023562484 title "End-Stage Renal Disease and the Practice of Nephrology" @default.
- W2023562484 cites W1984599074 @default.
- W2023562484 cites W1986792352 @default.
- W2023562484 cites W1987495678 @default.
- W2023562484 cites W2025252889 @default.
- W2023562484 cites W2066872483 @default.
- W2023562484 doi "https://doi.org/10.1016/s0272-6386(89)80174-x" @default.
- W2023562484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2816932" @default.
- W2023562484 hasPublicationYear "1989" @default.
- W2023562484 type Work @default.
- W2023562484 sameAs 2023562484 @default.
- W2023562484 citedByCount "12" @default.
- W2023562484 countsByYear W20235624842019 @default.
- W2023562484 crossrefType "journal-article" @default.
- W2023562484 hasAuthorship W2023562484A5004769568 @default.
- W2023562484 hasAuthorship W2023562484A5021680065 @default.
- W2023562484 hasAuthorship W2023562484A5024583991 @default.
- W2023562484 hasAuthorship W2023562484A5045047579 @default.
- W2023562484 hasAuthorship W2023562484A5049715758 @default.
- W2023562484 hasAuthorship W2023562484A5050217682 @default.
- W2023562484 hasAuthorship W2023562484A5051656604 @default.
- W2023562484 hasConcept C126322002 @default.
- W2023562484 hasConcept C142724271 @default.
- W2023562484 hasConcept C147483822 @default.
- W2023562484 hasConcept C159654299 @default.
- W2023562484 hasConcept C203014093 @default.
- W2023562484 hasConcept C2777529624 @default.
- W2023562484 hasConcept C2778236600 @default.
- W2023562484 hasConcept C2779026604 @default.
- W2023562484 hasConcept C2779102806 @default.
- W2023562484 hasConcept C2780091579 @default.
- W2023562484 hasConcept C2780368995 @default.
- W2023562484 hasConcept C2780898057 @default.
- W2023562484 hasConcept C2909524538 @default.
- W2023562484 hasConcept C2993140218 @default.
- W2023562484 hasConcept C30030713 @default.
- W2023562484 hasConcept C71924100 @default.
- W2023562484 hasConcept C87555872 @default.
- W2023562484 hasConceptScore W2023562484C126322002 @default.
- W2023562484 hasConceptScore W2023562484C142724271 @default.
- W2023562484 hasConceptScore W2023562484C147483822 @default.
- W2023562484 hasConceptScore W2023562484C159654299 @default.
- W2023562484 hasConceptScore W2023562484C203014093 @default.
- W2023562484 hasConceptScore W2023562484C2777529624 @default.
- W2023562484 hasConceptScore W2023562484C2778236600 @default.
- W2023562484 hasConceptScore W2023562484C2779026604 @default.
- W2023562484 hasConceptScore W2023562484C2779102806 @default.
- W2023562484 hasConceptScore W2023562484C2780091579 @default.
- W2023562484 hasConceptScore W2023562484C2780368995 @default.
- W2023562484 hasConceptScore W2023562484C2780898057 @default.
- W2023562484 hasConceptScore W2023562484C2909524538 @default.
- W2023562484 hasConceptScore W2023562484C2993140218 @default.
- W2023562484 hasConceptScore W2023562484C30030713 @default.
- W2023562484 hasConceptScore W2023562484C71924100 @default.
- W2023562484 hasConceptScore W2023562484C87555872 @default.
- W2023562484 hasIssue "5" @default.
- W2023562484 hasLocation W20235624841 @default.
- W2023562484 hasLocation W20235624842 @default.
- W2023562484 hasOpenAccess W2023562484 @default.
- W2023562484 hasPrimaryLocation W20235624841 @default.
- W2023562484 hasRelatedWork W1524068078 @default.
- W2023562484 hasRelatedWork W1931067834 @default.
- W2023562484 hasRelatedWork W2005833822 @default.
- W2023562484 hasRelatedWork W2013913419 @default.
- W2023562484 hasRelatedWork W2017447851 @default.
- W2023562484 hasRelatedWork W2078552237 @default.
- W2023562484 hasRelatedWork W2092538248 @default.
- W2023562484 hasRelatedWork W2415345273 @default.
- W2023562484 hasRelatedWork W2496762573 @default.
- W2023562484 hasRelatedWork W2029090008 @default.
- W2023562484 hasVolume "14" @default.
- W2023562484 isParatext "false" @default.
- W2023562484 isRetracted "false" @default.
- W2023562484 magId "2023562484" @default.
- W2023562484 workType "article" @default.