FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023573949> ?p ?o ?g. }

• W2023573949 endingPage "S61" @default.
• W2023573949 startingPage "S61" @default.
• W2023573949 abstract "Objective: EGF-R is expressed in the murine ovary and ligand (EGF, TGFa) activation plays a role in normal folliculogenesis. In this study, we investigated the effect of ovulation induction with exogenous gonadotropins on in vivo EGF-R expression in rat ovarian follicles during the estrous cycle.Design: Prospective, randomized, controlled murine study, approved by the animal IRB, at an academic institution.Materials/Methods: Adult female Wistar rats were housed on a 12-hour light/12-hour dark schedule. Estrous cycles were monitored by vaginal smears. Animals were separated into two groups: rats in the study group were injected intraperitoneally with 10 IU PMSG in early proestrus followed by 10 IU hCG 56 hours after PMSG; rats in the control group were injected with 0.9% saline only. Three animals in each group were euthanized according to their vaginal smears at late proestrus, estrus and metestrus. The ovaries were cryopreserved. Immunohistochemistry was performed using a monoclonal anti-EGF-R antibody (Clone no. 29.1, mouse IgG) and a peroxidase-labeled goat anti-mouse IgG, as the secondary antibody. A chromogenic reaction was developed by incubation with a solution of 3-Amino-9-ethylcarbazole for 15 minutes. All sections were counterstained with Mayer’s hematoxylin and evaluated by light microscopy. The intensity of EGF-R immunostaining in the ovarian follicles was assessed by using a computer-assisted image analysis and results were expressed as “pixels per area (ppa)”. Statistical analysis was done with the Wilcoxon test and ANOVA with post-hoc tests, where appropriate. P value of <0.05 was considered significant.Results: In late proestrus, there was a weak EGF-R expression in the control group, while moderate staining was seen in the study group (P = 0.06). During estrus, EGF-R expression significantly increased in the control group (p < 0.05 vs proestrus), while it decreased in the PMSG stimulated group (P = NS vs proestrus). EGF-R staining was lower during estrus in the PMSG stimulated group when compared to that in the control group (p < 0.05). Following ovulation, during metestrus, EGF-R expression decreased in the control group, although not significantly (P = NS, vs estrus), and it increased in the study group (p < 0.05, vs estrus). However, the EGF-R staining during metestrus was comparable between control and study groups (P = NS).Conclusions: Exogenous gonadotropin administration to cycling rats significantly modulates EGF-R expression in ovarian follicles in vivo when compared to cycling controls. We postulate that changes in EGF-R expression in the granulosa and theca cells during gonadotropin stimulation may modulate ligand (EGF and TGFa) tissue effects during folliculogenesis.Supported By: University of Illinois Campus Research Board, Chicago, Illinois Objective: EGF-R is expressed in the murine ovary and ligand (EGF, TGFa) activation plays a role in normal folliculogenesis. In this study, we investigated the effect of ovulation induction with exogenous gonadotropins on in vivo EGF-R expression in rat ovarian follicles during the estrous cycle. Design: Prospective, randomized, controlled murine study, approved by the animal IRB, at an academic institution. Materials/Methods: Adult female Wistar rats were housed on a 12-hour light/12-hour dark schedule. Estrous cycles were monitored by vaginal smears. Animals were separated into two groups: rats in the study group were injected intraperitoneally with 10 IU PMSG in early proestrus followed by 10 IU hCG 56 hours after PMSG; rats in the control group were injected with 0.9% saline only. Three animals in each group were euthanized according to their vaginal smears at late proestrus, estrus and metestrus. The ovaries were cryopreserved. Immunohistochemistry was performed using a monoclonal anti-EGF-R antibody (Clone no. 29.1, mouse IgG) and a peroxidase-labeled goat anti-mouse IgG, as the secondary antibody. A chromogenic reaction was developed by incubation with a solution of 3-Amino-9-ethylcarbazole for 15 minutes. All sections were counterstained with Mayer’s hematoxylin and evaluated by light microscopy. The intensity of EGF-R immunostaining in the ovarian follicles was assessed by using a computer-assisted image analysis and results were expressed as “pixels per area (ppa)”. Statistical analysis was done with the Wilcoxon test and ANOVA with post-hoc tests, where appropriate. P value of <0.05 was considered significant. Results: In late proestrus, there was a weak EGF-R expression in the control group, while moderate staining was seen in the study group (P = 0.06). During estrus, EGF-R expression significantly increased in the control group (p < 0.05 vs proestrus), while it decreased in the PMSG stimulated group (P = NS vs proestrus). EGF-R staining was lower during estrus in the PMSG stimulated group when compared to that in the control group (p < 0.05). Following ovulation, during metestrus, EGF-R expression decreased in the control group, although not significantly (P = NS, vs estrus), and it increased in the study group (p < 0.05, vs estrus). However, the EGF-R staining during metestrus was comparable between control and study groups (P = NS). Conclusions: Exogenous gonadotropin administration to cycling rats significantly modulates EGF-R expression in ovarian follicles in vivo when compared to cycling controls. We postulate that changes in EGF-R expression in the granulosa and theca cells during gonadotropin stimulation may modulate ligand (EGF and TGFa) tissue effects during folliculogenesis. Supported By: University of Illinois Campus Research Board, Chicago, Illinois" @default.
• W2023573949 created "2016-06-24" @default.
• W2023573949 creator A5000135680 @default.
• W2023573949 creator A5009754654 @default.
• W2023573949 creator A5026810367 @default.
• W2023573949 creator A5082270788 @default.
• W2023573949 date "2001-09-01" @default.
• W2023573949 modified "2023-09-26" @default.
• W2023573949 title "Effect of exogenous gonadotropins on in vivo epidermal growth factor receptor (EGF-R) expression in murine ovarian follicles throughout the estrous cycle." @default.
• W2023573949 doi "https://doi.org/10.1016/s0015-0282(01)02195-1" @default.
• W2023573949 hasPublicationYear "2001" @default.
• W2023573949 type Work @default.
• W2023573949 sameAs 2023573949 @default.
• W2023573949 citedByCount "0" @default.
• W2023573949 crossrefType "journal-article" @default.
• W2023573949 hasAuthorship W2023573949A5000135680 @default.
• W2023573949 hasAuthorship W2023573949A5009754654 @default.
• W2023573949 hasAuthorship W2023573949A5026810367 @default.
• W2023573949 hasAuthorship W2023573949A5082270788 @default.
• W2023573949 hasBestOaLocation W20235739491 @default.
• W2023573949 hasConcept C126322002 @default.
• W2023573949 hasConcept C134018914 @default.
• W2023573949 hasConcept C16685009 @default.
• W2023573949 hasConcept C170493617 @default.
• W2023573949 hasConcept C196843134 @default.
• W2023573949 hasConcept C1998276 @default.
• W2023573949 hasConcept C204232928 @default.
• W2023573949 hasConcept C2776362946 @default.
• W2023573949 hasConcept C2777702977 @default.
• W2023573949 hasConcept C2778324911 @default.
• W2023573949 hasConcept C4224716 @default.
• W2023573949 hasConcept C5512455 @default.
• W2023573949 hasConcept C71315377 @default.
• W2023573949 hasConcept C71924100 @default.
• W2023573949 hasConcept C86803240 @default.
• W2023573949 hasConcept C87073359 @default.
• W2023573949 hasConcept C95444343 @default.
• W2023573949 hasConceptScore W2023573949C126322002 @default.
• W2023573949 hasConceptScore W2023573949C134018914 @default.
• W2023573949 hasConceptScore W2023573949C16685009 @default.
• W2023573949 hasConceptScore W2023573949C170493617 @default.
• W2023573949 hasConceptScore W2023573949C196843134 @default.
• W2023573949 hasConceptScore W2023573949C1998276 @default.
• W2023573949 hasConceptScore W2023573949C204232928 @default.
• W2023573949 hasConceptScore W2023573949C2776362946 @default.
• W2023573949 hasConceptScore W2023573949C2777702977 @default.
• W2023573949 hasConceptScore W2023573949C2778324911 @default.
• W2023573949 hasConceptScore W2023573949C4224716 @default.
• W2023573949 hasConceptScore W2023573949C5512455 @default.
• W2023573949 hasConceptScore W2023573949C71315377 @default.
• W2023573949 hasConceptScore W2023573949C71924100 @default.
• W2023573949 hasConceptScore W2023573949C86803240 @default.
• W2023573949 hasConceptScore W2023573949C87073359 @default.
• W2023573949 hasConceptScore W2023573949C95444343 @default.
• W2023573949 hasIssue "3" @default.
• W2023573949 hasLocation W20235739491 @default.
• W2023573949 hasOpenAccess W2023573949 @default.
• W2023573949 hasPrimaryLocation W20235739491 @default.
• W2023573949 hasRelatedWork W1964469171 @default.
• W2023573949 hasRelatedWork W2018751547 @default.
• W2023573949 hasRelatedWork W2023197167 @default.
• W2023573949 hasRelatedWork W2047405877 @default.
• W2023573949 hasRelatedWork W2054166320 @default.
• W2023573949 hasRelatedWork W2086756948 @default.
• W2023573949 hasRelatedWork W2088569423 @default.
• W2023573949 hasRelatedWork W2110477606 @default.
• W2023573949 hasRelatedWork W2115122878 @default.
• W2023573949 hasRelatedWork W2169978493 @default.
• W2023573949 hasVolume "76" @default.
• W2023573949 isParatext "false" @default.
• W2023573949 isRetracted "false" @default.
• W2023573949 magId "2023573949" @default.
• W2023573949 workType "article" @default.