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- W2023579218 abstract "A high-resolution, two-dimensional Southern transfer method has been developed and was used to examine the distribution of a class of interspersed repeated sequences in human genomes. This method consists of two separate restriction enzyme digestions, including an in situ digestion, and two dimensional electrophoresis using a large-sized agarose gel. The first 163-base-pair region of the human LINE-1 full-length sequence was used to probe human genomic DNA from placental tissue samples. About 900 LlNE-1 signals were resolved from each DNA sample within a 2-D plane. The bulk of the fragments were between 0.5 to 23 kilobases in length. At a minimum 15 variant signals were detected between DNA samples from male and female individuals and at a minimum 16 variant signals were detected between two different female samples. This approach can potentially be used to perform high-resolution human genome fingerprinting analyses." @default.
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- W2023579218 date "1995-05-01" @default.
- W2023579218 modified "2023-09-26" @default.
- W2023579218 title "Two-Dimensional Electrophoresis Southern Transfer Method for Detecting Human Genome Variability Using a LINE-1 Sequence Probe" @default.
- W2023579218 doi "https://doi.org/10.1006/abio.1995.1287" @default.
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