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- W20235815 abstract "Two approaches to electrochemical immunoassay are reported.The first approach was an indirect method, involving anelectroactive, enzyme-catalysed, substrate to product reaction. Conditions were optimised for the amperometric detection of para-aminophenol, the electroactive product of the alkaline phosphatase catalysed hydrolysis of a new substrate, p-aminophenylphosphate, after separation by HPLC.The second approach involved the direct electrochemicaldetection of an immunoglobulin species, IgG, and theelectrochemical monitoring of the immunological interaction.In an attempt to characterise the electrochemical response of such proteins, studies were carried out on the adsorptivestripping voltammetry of the immunoglobulin fragments,F(ab1 )2 and Fab.The globular proteins avidin and streptavidin, were thenstudied as these were similarly constructed proteins, exceptavidin had a disulphide bond, and streptavidin has not. Theinteraction of avidin and streptavidin with biotinylated IgGwas also reported as this is an important labelling procedure in immunoassay." @default.
- W20235815 created "2016-06-24" @default.
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- W20235815 date "1989-01-01" @default.
- W20235815 modified "2023-09-27" @default.
- W20235815 title "Studies on direct and indirect electrochemical immunoassays" @default.
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