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- W2023582428 abstract "The ColE2 DNA can be replicated in an in vitro system consisting of a crude extract of Escherichia coli cells. DNA synthesis requires a plasmid-coded protein (Rep) and host DNA polymerase I but not host RNA polymerase. Replication starts at a fixed region containing the origin and proceeds unidirectionally. The leading- and lagging-strand DNA fragments synthesized around the origin were identified from early replicative intermediates. The 5' end of the leading-strand DNA fragment was mapped at a unique position in the minimal origin and carried RNA of a few residues. The results suggested that the initiation of the leading-strand DNA synthesis does not require the host DnaG protein. Thus the Rep protein itself seems to be a primase. Synthesis of the primer RNA at a fixed site in the origin region on a double-stranded DNA template is a unique property of the ColE2 Rep protein among other known primases. The 3' end of the lagging-strand DNA fragment was mapped at a unique position just at the end of the minimal origin region. Termination of the lagging-strand DNA fragment at that position seems to be the mechanism of the unidirectional replication of ColE2 plasmid." @default.
- W2023582428 created "2016-06-24" @default.
- W2023582428 creator A5010004241 @default.
- W2023582428 creator A5015256874 @default.
- W2023582428 date "1995-01-01" @default.
- W2023582428 modified "2023-09-25" @default.
- W2023582428 title "Initiation of unidirectional CoIE2 DNA replication by a unique priming mechanism" @default.
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- W2023582428 doi "https://doi.org/10.1093/nar/23.20.4196" @default.
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