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- W2023583075 abstract "The serotonin1A (5-HT1A) receptors are members of a superfamily of seven transmembrane domain receptors that couple to G-proteins. They appear to be involved in various behavioural and cognitive functions. This paper reports an efficient strategy to solubilize 5-HT1A receptors from bovine hippocampal membranes using the zwitterionic detergent CHAPS which is mild and non-denaturing. Since high concentration of CHAPS has earlier been shown to induce dissociation and depletion of G-protein sub-units, a low (pre-micellar) concentration of CHAPS was used for solubilizing 5-HT1A receptors in the presence of NaCl followed by PEG precipitation. This results in solubilization of 5-HT1A receptors with a high degree of efficiency and gives rise to high affinity, functionally active G-protein-sensitive solubilized receptors. Optimal solubilization of the receptor from the native source with high ligand binding affinity and intact signal transduction components may constitute the first step in the molecular characterization of the 5-HT1A receptor in particular, and G-protein-coupled receptors in general." @default.
- W2023583075 created "2016-06-24" @default.
- W2023583075 creator A5006698991 @default.
- W2023583075 creator A5063835467 @default.
- W2023583075 creator A5079748663 @default.
- W2023583075 date "2002-01-01" @default.
- W2023583075 modified "2023-10-16" @default.
- W2023583075 title "Solubilization of high affinity G-protein-coupled serotonin 1A receptors from bovine hippocampus using pre-micellar CHAPS at low concentration" @default.
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- W2023583075 doi "https://doi.org/10.1080/09687680210149586" @default.
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