FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023587437> ?p ?o ?g. }

• W2023587437 endingPage "661" @default.
• W2023587437 startingPage "655" @default.
• W2023587437 abstract "Ionotropic glutamate receptors (iGluRs) are responsible for extracellular signaling in the central nervous system. However, the relationship between the overall structure of the protein and its function has yet to be resolved. Atomic force microscopy (AFM) is an important technique that allows nano-scale imaging in liquid. In the present work we have succeeded in imaging by AFM of the external features of the most common iGluR, AMPA-R (α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor), in a physiological environment. Homomeric GluR3 receptors were over-expressed in insect cells, purified and reconstituted into lipid membranes. AFM images were obtained in a buffer from membranes immobilized on a mica substrate. Using Au nanoparticle-conjugated antibodies, we show that proteins reconstitute predominantly with the N-terminal domain uppermost on the membrane. A tetrameric receptor structure is clearly observed, but it displays considerable heterogeneity, and the dimensions differ considerably from cryo-electron microscopy measurements. Our results indicate that the extracellular domains of AMPA-R are highly flexible in a physiological environment. AFM allows us to observe the protein surface structure, suggesting the possibility of visualizing real time conformational changes of a functioning protein. This knowledge may be useful for neuroscience as well as in pharmaceutical applications." @default.
• W2023587437 created "2016-06-24" @default.
• W2023587437 creator A5000417874 @default.
• W2023587437 creator A5017543385 @default.
• W2023587437 creator A5062099923 @default.
• W2023587437 creator A5065486427 @default.
• W2023587437 creator A5068296347 @default.
• W2023587437 creator A5077192079 @default.
• W2023587437 date "2010-07-01" @default.
• W2023587437 modified "2023-10-18" @default.
• W2023587437 title "AFM observation of single, functioning ionotropic glutamate receptors reconstituted in lipid bilayers" @default.
• W2023587437 cites W1492420763 @default.
• W2023587437 cites W1565999785 @default.
• W2023587437 cites W1586914066 @default.
• W2023587437 cites W1908222510 @default.
• W2023587437 cites W1963733435 @default.
• W2023587437 cites W1971402629 @default.
• W2023587437 cites W1982215124 @default.
• W2023587437 cites W1990567099 @default.
• W2023587437 cites W1990722516 @default.
• W2023587437 cites W1996015681 @default.
• W2023587437 cites W1996208391 @default.
• W2023587437 cites W1997126666 @default.
• W2023587437 cites W1999968276 @default.
• W2023587437 cites W2000249949 @default.
• W2023587437 cites W2009467796 @default.
• W2023587437 cites W2010747985 @default.
• W2023587437 cites W2012678641 @default.
• W2023587437 cites W2013973914 @default.
• W2023587437 cites W2017428898 @default.
• W2023587437 cites W2023108057 @default.
• W2023587437 cites W2023626807 @default.
• W2023587437 cites W2037773086 @default.
• W2023587437 cites W2060732248 @default.
• W2023587437 cites W2066358338 @default.
• W2023587437 cites W2079302576 @default.
• W2023587437 cites W2089792637 @default.
• W2023587437 cites W2092237780 @default.
• W2023587437 cites W2092665603 @default.
• W2023587437 cites W2093532462 @default.
• W2023587437 cites W2100880723 @default.
• W2023587437 cites W2109228055 @default.
• W2023587437 cites W2135635018 @default.
• W2023587437 cites W2142859342 @default.
• W2023587437 cites W2170473509 @default.
• W2023587437 cites W2171021618 @default.
• W2023587437 cites W659295882 @default.
• W2023587437 doi "https://doi.org/10.1016/j.bbagen.2010.03.002" @default.
• W2023587437 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20307628" @default.
• W2023587437 hasPublicationYear "2010" @default.
• W2023587437 type Work @default.
• W2023587437 sameAs 2023587437 @default.
• W2023587437 citedByCount "10" @default.
• W2023587437 countsByYear W20235874372012 @default.
• W2023587437 countsByYear W20235874372013 @default.
• W2023587437 countsByYear W20235874372014 @default.
• W2023587437 countsByYear W20235874372016 @default.
• W2023587437 crossrefType "journal-article" @default.
• W2023587437 hasAuthorship W2023587437A5000417874 @default.
• W2023587437 hasAuthorship W2023587437A5017543385 @default.
• W2023587437 hasAuthorship W2023587437A5062099923 @default.
• W2023587437 hasAuthorship W2023587437A5065486427 @default.
• W2023587437 hasAuthorship W2023587437A5068296347 @default.
• W2023587437 hasAuthorship W2023587437A5077192079 @default.
• W2023587437 hasConcept C104292427 @default.
• W2023587437 hasConcept C104317684 @default.
• W2023587437 hasConcept C115955781 @default.
• W2023587437 hasConcept C12554922 @default.
• W2023587437 hasConcept C160268369 @default.
• W2023587437 hasConcept C170493617 @default.
• W2023587437 hasConcept C185592680 @default.
• W2023587437 hasConcept C2776104367 @default.
• W2023587437 hasConcept C2781001490 @default.
• W2023587437 hasConcept C28406088 @default.
• W2023587437 hasConcept C39944091 @default.
• W2023587437 hasConcept C41625074 @default.
• W2023587437 hasConcept C55493867 @default.
• W2023587437 hasConcept C61174792 @default.
• W2023587437 hasConcept C86803240 @default.
• W2023587437 hasConceptScore W2023587437C104292427 @default.
• W2023587437 hasConceptScore W2023587437C104317684 @default.
• W2023587437 hasConceptScore W2023587437C115955781 @default.
• W2023587437 hasConceptScore W2023587437C12554922 @default.
• W2023587437 hasConceptScore W2023587437C160268369 @default.
• W2023587437 hasConceptScore W2023587437C170493617 @default.
• W2023587437 hasConceptScore W2023587437C185592680 @default.
• W2023587437 hasConceptScore W2023587437C2776104367 @default.
• W2023587437 hasConceptScore W2023587437C2781001490 @default.
• W2023587437 hasConceptScore W2023587437C28406088 @default.
• W2023587437 hasConceptScore W2023587437C39944091 @default.
• W2023587437 hasConceptScore W2023587437C41625074 @default.
• W2023587437 hasConceptScore W2023587437C55493867 @default.
• W2023587437 hasConceptScore W2023587437C61174792 @default.
• W2023587437 hasConceptScore W2023587437C86803240 @default.
• W2023587437 hasIssue "7" @default.
• W2023587437 hasLocation W20235874371 @default.
• W2023587437 hasLocation W20235874372 @default.
• W2023587437 hasOpenAccess W2023587437 @default.

• next