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- W2023588431 abstract "Double digestion of oligonucleotides obtained from ribonuclease T1 or pancreatic ribonuclease A fingerprints results in the following series of products: (Ap)0-nCp, (Ap)0-nUp, and (Ap)0-nGp. A new micromethod is described for the rapid analysis of these digests. The procedure consists of two-dimensional chromatography on a small PEI thin-layer plate. In the first dimension, the oligonucleotides are separated independent of their Ap content into three groups, which represent the Cp-, Gp-, and Up- 3′-terminal oligonucleotide series, respectively. In the second dimension, the products are fractionated according to their chain length. This method, which allows direct identification of even the longer Ap tracts in a double digest of an oligonucleotide or an RNA chain, is very rapid and highly sensitive and can be applied to the simultaneous analysis of a large number of samples in a single run. The procedure has also been adapted to the analysis of pancreatic ribonuclease A digests of small RNA fragments." @default.
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- W2023588431 date "1977-11-01" @default.
- W2023588431 modified "2023-09-26" @default.
- W2023588431 title "Micro thin-layer techniques for rapid sequence analysis of 32P-labeled RNA: Double digestion and pancreatic ribonuclease analyses" @default.
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- W2023588431 doi "https://doi.org/10.1016/0003-2697(77)90531-0" @default.
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