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- W2023596735 abstract "Abstract Chromatography on benzoylated naphthoylated DEAE-cellulose (BN-cellulose) is indicated to be a procedure sensitive within certain limits to the degree of single-strandedness in a double-stranded DNA molecule. For linear coliphage P22 DNA digested with λ-exonuclease to the extent of 0.0–5.5 %, there was a linear relationship between the degree of digestion and the concentration of caffeine at which peak elution of the digested DNA occurred from BN-cellulose. This relationship has been used to obtain an estimate of the size of single-stranded regions of DNA generated in bacteria as a consequence of replication on a template bearing thymine dimers. The experiments indicate that BN-cellulose may be successfully exploited to purify this form of DNA." @default.
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- W2023596735 date "1971-01-01" @default.
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- W2023596735 title "Usefulness of benzoylated naphthoylated DEAE-cellulose to distinguish and fractionate double-stranded DNA bearing different extents of single-stranded regions" @default.
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- W2023596735 doi "https://doi.org/10.1016/0005-2787(71)90551-x" @default.
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