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- W2023597066 abstract "We treated an 11-year-old boy with a testicular Leydig cell tumor. We analyzed the testosterone production of this tumor by immunolocalization of steroidogenic enzymes and in vitro three-dimensional histoculture. Spermatic venous blood from the tumor bearing testis had noticeably high concentrations of testosterone and androstenedione. The tumor had the characteristic ultras-tructural features of steroid producing cells and was immunoreactive for P450scc (side chain cleavage), 3βHSD (hydroxysteroid dehydrogenase) and P450c17 (17α-hydroxylase). Three-dimensional collagengel-supported histoculture demonstrated that the tumor tissue in the culture maintained its histologic architecture, expression of steroidogenic enzymes, and secretion of testosterone into the medium for up to 7 days in culture. Histoculture preserved in vitro testosterone production in this case of testicular Leydig cell tumor." @default.
- W2023597066 created "2016-06-24" @default.
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- W2023597066 date "1996-01-01" @default.
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- W2023597066 title "Leydig Cell Tumor of the Testis: Analysis of Testosterone Production and Secretion by Three-Dimensional Histoculture." @default.
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- W2023597066 doi "https://doi.org/10.1507/endocrj.43.73" @default.
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