FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023597568> ?p ?o ?g. }

• W2023597568 endingPage "e16576" @default.
• W2023597568 startingPage "e16576" @default.
• W2023597568 abstract "The extensive use of avidin and streptavidin in life sciences originates from the extraordinary tight biotin-binding affinity of these tetrameric proteins. Numerous studies have been performed to modify the biotin-binding affinity of (strept)avidin to improve the existing applications. Even so, (strept)avidin greatly favours its natural ligand, biotin. Here we engineered the biotin-binding pocket of avidin with a single point mutation S16C and thus introduced a chemically active thiol group, which could be covalently coupled with thiol-reactive molecules. This approach was applied to the previously reported bivalent dual chain avidin by modifying one binding site while preserving the other one intact. Maleimide was then coupled to the modified binding site resulting in a decrease in biotin affinity. Furthermore, we showed that this thiol could be covalently coupled to other maleimide derivatives, for instance fluorescent labels, allowing intratetrameric FRET. The bifunctional avidins described here provide improved and novel tools for applications such as the biofunctionalization of surfaces." @default.
• W2023597568 created "2016-06-24" @default.
• W2023597568 creator A5037038554 @default.
• W2023597568 creator A5040591934 @default.
• W2023597568 creator A5045623177 @default.
• W2023597568 creator A5052387458 @default.
• W2023597568 creator A5058530904 @default.
• W2023597568 creator A5064662050 @default.
• W2023597568 creator A5068521951 @default.
• W2023597568 creator A5086433341 @default.
• W2023597568 date "2011-01-27" @default.
• W2023597568 modified "2023-10-18" @default.
• W2023597568 title "Bifunctional Avidin with Covalently Modifiable Ligand Binding Site" @default.
• W2023597568 cites W1510114475 @default.
• W2023597568 cites W1968056768 @default.
• W2023597568 cites W1971288359 @default.
• W2023597568 cites W1981309659 @default.
• W2023597568 cites W1985061567 @default.
• W2023597568 cites W1988774285 @default.
• W2023597568 cites W1993599314 @default.
• W2023597568 cites W2011986444 @default.
• W2023597568 cites W2013966476 @default.
• W2023597568 cites W2016366290 @default.
• W2023597568 cites W2021093384 @default.
• W2023597568 cites W2027836552 @default.
• W2023597568 cites W2037546764 @default.
• W2023597568 cites W2037576778 @default.
• W2023597568 cites W2041094231 @default.
• W2023597568 cites W2041096731 @default.
• W2023597568 cites W2059910533 @default.
• W2023597568 cites W2060824204 @default.
• W2023597568 cites W2065434897 @default.
• W2023597568 cites W2075719564 @default.
• W2023597568 cites W2078224854 @default.
• W2023597568 cites W2082867124 @default.
• W2023597568 cites W2111197146 @default.
• W2023597568 cites W2123285901 @default.
• W2023597568 cites W2125813323 @default.
• W2023597568 cites W2151492765 @default.
• W2023597568 cites W2158332589 @default.
• W2023597568 cites W2287832430 @default.
• W2023597568 cites W4211027922 @default.
• W2023597568 doi "https://doi.org/10.1371/journal.pone.0016576" @default.
• W2023597568 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3029397" @default.
• W2023597568 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21305032" @default.
• W2023597568 hasPublicationYear "2011" @default.
• W2023597568 type Work @default.
• W2023597568 sameAs 2023597568 @default.
• W2023597568 citedByCount "16" @default.
• W2023597568 countsByYear W20235975682012 @default.
• W2023597568 countsByYear W20235975682014 @default.
• W2023597568 countsByYear W20235975682015 @default.
• W2023597568 countsByYear W20235975682016 @default.
• W2023597568 countsByYear W20235975682019 @default.
• W2023597568 countsByYear W20235975682020 @default.
• W2023597568 countsByYear W20235975682021 @default.
• W2023597568 countsByYear W20235975682023 @default.
• W2023597568 crossrefType "journal-article" @default.
• W2023597568 hasAuthorship W2023597568A5037038554 @default.
• W2023597568 hasAuthorship W2023597568A5040591934 @default.
• W2023597568 hasAuthorship W2023597568A5045623177 @default.
• W2023597568 hasAuthorship W2023597568A5052387458 @default.
• W2023597568 hasAuthorship W2023597568A5058530904 @default.
• W2023597568 hasAuthorship W2023597568A5064662050 @default.
• W2023597568 hasAuthorship W2023597568A5068521951 @default.
• W2023597568 hasAuthorship W2023597568A5086433341 @default.
• W2023597568 hasBestOaLocation W20235975681 @default.
• W2023597568 hasConcept C107824862 @default.
• W2023597568 hasConcept C116569031 @default.
• W2023597568 hasConcept C121332964 @default.
• W2023597568 hasConcept C161790260 @default.
• W2023597568 hasConcept C170493617 @default.
• W2023597568 hasConcept C176406525 @default.
• W2023597568 hasConcept C178790620 @default.
• W2023597568 hasConcept C180577832 @default.
• W2023597568 hasConcept C181199279 @default.
• W2023597568 hasConcept C185592680 @default.
• W2023597568 hasConcept C188027245 @default.
• W2023597568 hasConcept C204628709 @default.
• W2023597568 hasConcept C21951064 @default.
• W2023597568 hasConcept C2776190903 @default.
• W2023597568 hasConcept C2778204606 @default.
• W2023597568 hasConcept C2779201268 @default.
• W2023597568 hasConcept C2780105360 @default.
• W2023597568 hasConcept C2780443040 @default.
• W2023597568 hasConcept C2781324293 @default.
• W2023597568 hasConcept C55493867 @default.
• W2023597568 hasConcept C62520636 @default.
• W2023597568 hasConcept C91881484 @default.
• W2023597568 hasConcept C96305047 @default.
• W2023597568 hasConceptScore W2023597568C107824862 @default.
• W2023597568 hasConceptScore W2023597568C116569031 @default.
• W2023597568 hasConceptScore W2023597568C121332964 @default.
• W2023597568 hasConceptScore W2023597568C161790260 @default.
• W2023597568 hasConceptScore W2023597568C170493617 @default.
• W2023597568 hasConceptScore W2023597568C176406525 @default.
• W2023597568 hasConceptScore W2023597568C178790620 @default.
• W2023597568 hasConceptScore W2023597568C180577832 @default.

• next