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- W2023598145 abstract "An enzyme-linked immunosorbent assay for urinary 6-β-hydroxycortisol (6-β-OHF) was developed. A highly specific antiserum against 6-β-OHF-21-hemisuccinate bovine serum albumin (6-β-OHF-21-HS-BSA) raised in rabbit was used. The enzyme-labelled hapten was prepared by the mixed anhydride method using horse radish peroxidase (HRP). The limit of detection is very low (10 pg), the intra-assay variation ranges from 5.2–6.6%, the inter-assay variation is 8.2–8.3%. Cross reactivities of various steroids are very low. The diurnal variations in urinary 6-β-OHF and it's ratio to 17-hydroxycortico-steroids (17-OHCS) were studied in six healthy volunteers. No significant difference was found within the 24 h for the ratio of 6-β-OHF to 17-OHCS. A large increase of 8–12 h urinary 6-β-OHF was observed in patients receiving chronic treatment with antiepileptics." @default.
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- W2023598145 title "ELISA of 6-beta-hydroxycortisol in human urine: Diurnal variations and effects of antiepileptic therapy" @default.
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- W2023598145 doi "https://doi.org/10.1016/0009-8981(86)90302-5" @default.
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