FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023608085> ?p ?o ?g. }

• W2023608085 endingPage "12916" @default.
• W2023608085 startingPage "12910" @default.
• W2023608085 abstract "Rational drug design depends on the knowledge of the three-dimensional (3D) structure of complexes between proteins and lead compounds of low molecular weight. A novel nuclear magnetic resonance (NMR) spectroscopy strategy based on the paramagnetic effects from lanthanide ions allows the rapid determination of the 3D structure of a small ligand molecule bound to its protein target in solution and, simultaneously, its location and orientation with respect to the protein. The method relies on the presence of a lanthanide ion in the protein target and on fast exchange between bound and free ligand. The binding affinity of the ligand and the paramagnetic effects experienced in the bound state are derived from concentration-dependent 1H and 13C spectra of the ligand at natural isotopic abundance. Combined with prior knowledge of the crystal or solution structure of the protein and of the magnetic susceptibility tensor of the lanthanide ion, the paramagnetic data define the location and orientation of the bound ligand molecule with respect to the protein from simple 1D NMR spectra. The method was verified with the ternary 30 kDa complex between the lanthanide-labeled N-terminal domain of the ε exonuclease subunit from the Escherichia coli DNA polymerase III, the subunit θ, and thymidine. The binding mode of thymidine was found to be very similar to that of thymidine monophosphate present in the crystal structure." @default.
• W2023608085 created "2016-06-24" @default.
• W2023608085 creator A5011359890 @default.
• W2023608085 creator A5027778630 @default.
• W2023608085 creator A5036604197 @default.
• W2023608085 creator A5061859662 @default.
• W2023608085 creator A5066355611 @default.
• W2023608085 date "2006-09-13" @default.
• W2023608085 modified "2023-10-12" @default.
• W2023608085 title "Structure Determination of Protein−Ligand Complexes by Transferred Paramagnetic Shifts" @default.
• W2023608085 cites W1968877288 @default.
• W2023608085 cites W1970911164 @default.
• W2023608085 cites W1971247331 @default.
• W2023608085 cites W1972459558 @default.
• W2023608085 cites W1975953204 @default.
• W2023608085 cites W1980685106 @default.
• W2023608085 cites W1985913381 @default.
• W2023608085 cites W1990815548 @default.
• W2023608085 cites W1996263865 @default.
• W2023608085 cites W2011397327 @default.
• W2023608085 cites W2022471410 @default.
• W2023608085 cites W2035103565 @default.
• W2023608085 cites W2038895598 @default.
• W2023608085 cites W2049661691 @default.
• W2023608085 cites W2051545337 @default.
• W2023608085 cites W2057981872 @default.
• W2023608085 cites W2058048068 @default.
• W2023608085 cites W2058616804 @default.
• W2023608085 cites W2076575095 @default.
• W2023608085 cites W2081281861 @default.
• W2023608085 cites W2087633444 @default.
• W2023608085 cites W2090074167 @default.
• W2023608085 cites W2090338420 @default.
• W2023608085 cites W2135010069 @default.
• W2023608085 cites W2138031164 @default.
• W2023608085 cites W2158010269 @default.
• W2023608085 cites W2166066662 @default.
• W2023608085 cites W2170671445 @default.
• W2023608085 cites W2951254503 @default.
• W2023608085 doi "https://doi.org/10.1021/ja063584z" @default.
• W2023608085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17002387" @default.
• W2023608085 hasPublicationYear "2006" @default.
• W2023608085 type Work @default.
• W2023608085 sameAs 2023608085 @default.
• W2023608085 citedByCount "96" @default.
• W2023608085 countsByYear W20236080852012 @default.
• W2023608085 countsByYear W20236080852013 @default.
• W2023608085 countsByYear W20236080852014 @default.
• W2023608085 countsByYear W20236080852015 @default.
• W2023608085 countsByYear W20236080852016 @default.
• W2023608085 countsByYear W20236080852017 @default.
• W2023608085 countsByYear W20236080852018 @default.
• W2023608085 countsByYear W20236080852019 @default.
• W2023608085 countsByYear W20236080852020 @default.
• W2023608085 countsByYear W20236080852021 @default.
• W2023608085 countsByYear W20236080852022 @default.
• W2023608085 countsByYear W20236080852023 @default.
• W2023608085 crossrefType "journal-article" @default.
• W2023608085 hasAuthorship W2023608085A5011359890 @default.
• W2023608085 hasAuthorship W2023608085A5027778630 @default.
• W2023608085 hasAuthorship W2023608085A5036604197 @default.
• W2023608085 hasAuthorship W2023608085A5061859662 @default.
• W2023608085 hasAuthorship W2023608085A5066355611 @default.
• W2023608085 hasConcept C116569031 @default.
• W2023608085 hasConcept C121332964 @default.
• W2023608085 hasConcept C124712363 @default.
• W2023608085 hasConcept C145148216 @default.
• W2023608085 hasConcept C170493617 @default.
• W2023608085 hasConcept C178790620 @default.
• W2023608085 hasConcept C181199279 @default.
• W2023608085 hasConcept C185592680 @default.
• W2023608085 hasConcept C201770740 @default.
• W2023608085 hasConcept C32909587 @default.
• W2023608085 hasConcept C55493867 @default.
• W2023608085 hasConcept C62520636 @default.
• W2023608085 hasConcept C66974803 @default.
• W2023608085 hasConcept C71240020 @default.
• W2023608085 hasConcept C8010536 @default.
• W2023608085 hasConcept C86756495 @default.
• W2023608085 hasConceptScore W2023608085C116569031 @default.
• W2023608085 hasConceptScore W2023608085C121332964 @default.
• W2023608085 hasConceptScore W2023608085C124712363 @default.
• W2023608085 hasConceptScore W2023608085C145148216 @default.
• W2023608085 hasConceptScore W2023608085C170493617 @default.
• W2023608085 hasConceptScore W2023608085C178790620 @default.
• W2023608085 hasConceptScore W2023608085C181199279 @default.
• W2023608085 hasConceptScore W2023608085C185592680 @default.
• W2023608085 hasConceptScore W2023608085C201770740 @default.
• W2023608085 hasConceptScore W2023608085C32909587 @default.
• W2023608085 hasConceptScore W2023608085C55493867 @default.
• W2023608085 hasConceptScore W2023608085C62520636 @default.
• W2023608085 hasConceptScore W2023608085C66974803 @default.
• W2023608085 hasConceptScore W2023608085C71240020 @default.
• W2023608085 hasConceptScore W2023608085C8010536 @default.
• W2023608085 hasConceptScore W2023608085C86756495 @default.
• W2023608085 hasIssue "39" @default.
• W2023608085 hasLocation W20236080851 @default.
• W2023608085 hasLocation W20236080852 @default.

• next