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- W2023625201 abstract "Gene transfer into CNS is critical for potential therapeutic applications as well as for the study of the genetic basis of neural development and nerve function. Unfortunately, lipid-based gene transfer to CNS cells is extremely inefficient since the nucleus of these post-mitotic cells presents a significant barrier to transfection. We report the development of a simple and highly efficient lipofection method for primary embryonic rat hippocampal neurons (up to 25% transfection) that exploits the M9 sequence of the non-classical nuclear localization signal of heterogeneous nuclear ribonucleoprotein A1 for targeting β2-karyopherin (transportin-1). M9-assistant lipofection resulted in 20–100-fold enhancement of transfection over lipofection alone for embryonic-derived retinal ganglion cells, rat pheochromocytoma (PC12) cells, embryonic rat ventral mesencephalon neurons, as well as the clinically relevant human NT2 cells or retinoic acid-differentiated NT2 neurons. This technique can facilitate the implementation of promoter construct experiments in post-mitotic cells, stable transformant generation, and dominant-negative mutant expression techniques in CNS cells." @default.
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- W2023625201 date "2002-06-01" @default.
- W2023625201 modified "2023-09-27" @default.
- W2023625201 title "Non-classical nuclear localization signal peptides for high efficiency lipofection of primary neurons and neuronal cell lines" @default.
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- W2023625201 doi "https://doi.org/10.1016/s0306-4522(02)00044-1" @default.
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