Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023641472> ?p ?o ?g. }
- W2023641472 endingPage "5950" @default.
- W2023641472 startingPage "5940" @default.
- W2023641472 abstract "Excessive cathepsin K (catK)-mediated turnover of fibrillar type I and II collagens in bone and cartilage leads to osteoporosis and osteoarthritis. However, little is known about how catK degrades compact collagen macromolecules. The present study is aimed to explore the structural and mechanical consequences of collagen fiber degradation by catK. Mouse tail type I collagen fibers were incubated with either catK or non-collagenase cathepsins. Methods used include scanning electron microscopy, protein electrophoresis, atomic force microscopy, and tensile strength testing. Our study revealed evidence of proteoglycan network degradation, followed by the progressive disassembly of macroscopic collagen fibers into primary structural elements by catK. Proteolytically released GAGs are involved in the generation of collagenolytically active catK-GAG complexes as shown by AFM. In addition to their structural disintegration, a decrease in the tensile properties of fibers was observed due to the action of catK. The Young's moduli of untreated collagen fibers versus catK-treated fibers in dehydrated conditions were 3.2 ± 0.68 GPa and 1.9 ± 0.65 GPa, respectively. In contrast, cathepsin L, V, B, and S revealed no collagenase activity, except the disruption of proteoglycan-GAG interfibrillar bridges, which slightly decreased the tensile strength of fibers." @default.
- W2023641472 created "2016-06-24" @default.
- W2023641472 creator A5002173231 @default.
- W2023641472 creator A5008405644 @default.
- W2023641472 creator A5033020063 @default.
- W2023641472 creator A5049350842 @default.
- W2023641472 creator A5064961267 @default.
- W2023641472 creator A5077462305 @default.
- W2023641472 creator A5078725430 @default.
- W2023641472 date "2013-02-01" @default.
- W2023641472 modified "2023-10-17" @default.
- W2023641472 title "Effects of Cysteine Proteases on the Structural and Mechanical Properties of Collagen Fibers" @default.
- W2023641472 cites W1484740986 @default.
- W2023641472 cites W1515227258 @default.
- W2023641472 cites W1787865208 @default.
- W2023641472 cites W1812339273 @default.
- W2023641472 cites W1968024679 @default.
- W2023641472 cites W1968329347 @default.
- W2023641472 cites W1972212033 @default.
- W2023641472 cites W1974224855 @default.
- W2023641472 cites W1978263865 @default.
- W2023641472 cites W1979495400 @default.
- W2023641472 cites W1986137297 @default.
- W2023641472 cites W1989560280 @default.
- W2023641472 cites W1991248456 @default.
- W2023641472 cites W1991947705 @default.
- W2023641472 cites W1995489138 @default.
- W2023641472 cites W1995489201 @default.
- W2023641472 cites W1996266076 @default.
- W2023641472 cites W1996401487 @default.
- W2023641472 cites W2002192590 @default.
- W2023641472 cites W2006977892 @default.
- W2023641472 cites W2017271806 @default.
- W2023641472 cites W2017614987 @default.
- W2023641472 cites W2020548070 @default.
- W2023641472 cites W2038005900 @default.
- W2023641472 cites W2043371368 @default.
- W2023641472 cites W2049891971 @default.
- W2023641472 cites W2052717554 @default.
- W2023641472 cites W2054666938 @default.
- W2023641472 cites W2056540680 @default.
- W2023641472 cites W2057635936 @default.
- W2023641472 cites W2067439258 @default.
- W2023641472 cites W2071468233 @default.
- W2023641472 cites W2073397502 @default.
- W2023641472 cites W2079583888 @default.
- W2023641472 cites W2090128986 @default.
- W2023641472 cites W2097707959 @default.
- W2023641472 cites W2099885273 @default.
- W2023641472 cites W2109350686 @default.
- W2023641472 cites W2113950718 @default.
- W2023641472 cites W2118720090 @default.
- W2023641472 cites W2120237177 @default.
- W2023641472 cites W2127251407 @default.
- W2023641472 cites W2141514409 @default.
- W2023641472 cites W2143659399 @default.
- W2023641472 cites W2148476302 @default.
- W2023641472 cites W2150238384 @default.
- W2023641472 cites W2169685756 @default.
- W2023641472 cites W2293647140 @default.
- W2023641472 cites W2954701605 @default.
- W2023641472 cites W4361983513 @default.
- W2023641472 doi "https://doi.org/10.1074/jbc.m112.419689" @default.
- W2023641472 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3581366" @default.
- W2023641472 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23297404" @default.
- W2023641472 hasPublicationYear "2013" @default.
- W2023641472 type Work @default.
- W2023641472 sameAs 2023641472 @default.
- W2023641472 citedByCount "75" @default.
- W2023641472 countsByYear W20236414722013 @default.
- W2023641472 countsByYear W20236414722014 @default.
- W2023641472 countsByYear W20236414722015 @default.
- W2023641472 countsByYear W20236414722016 @default.
- W2023641472 countsByYear W20236414722017 @default.
- W2023641472 countsByYear W20236414722018 @default.
- W2023641472 countsByYear W20236414722019 @default.
- W2023641472 countsByYear W20236414722020 @default.
- W2023641472 countsByYear W20236414722021 @default.
- W2023641472 countsByYear W20236414722022 @default.
- W2023641472 countsByYear W20236414722023 @default.
- W2023641472 crossrefType "journal-article" @default.
- W2023641472 hasAuthorship W2023641472A5002173231 @default.
- W2023641472 hasAuthorship W2023641472A5008405644 @default.
- W2023641472 hasAuthorship W2023641472A5033020063 @default.
- W2023641472 hasAuthorship W2023641472A5049350842 @default.
- W2023641472 hasAuthorship W2023641472A5064961267 @default.
- W2023641472 hasAuthorship W2023641472A5077462305 @default.
- W2023641472 hasAuthorship W2023641472A5078725430 @default.
- W2023641472 hasBestOaLocation W20236414721 @default.
- W2023641472 hasConcept C101990758 @default.
- W2023641472 hasConcept C105702510 @default.
- W2023641472 hasConcept C112950240 @default.
- W2023641472 hasConcept C12554922 @default.
- W2023641472 hasConcept C134018914 @default.
- W2023641472 hasConcept C155181932 @default.
- W2023641472 hasConcept C159985019 @default.
- W2023641472 hasConcept C167844969 @default.
- W2023641472 hasConcept C181199279 @default.