Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023642742> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2023642742 abstract "Abnormal prion protein (PrP Sc ) is highly resistant to inactivation by conventional chemical and physical means. This study was to determine if microbes from the environment could be used to degrade PrP Sc in central nervous system (CNS) tissues from scrapie positive sheep as measured by Western blot. In the first experiment, the number of microbes in CNS tissue suspended in saline was reduced by autoclaving the suspension at 121°C for 5 minutes. Aliquots of this preparation were then inoculated with additional ovine fecal microbes and controls were not inoculated. The results showed that the addition of microbes increased the degradation of PrP Sc in specimens during incubation at room temperature (RT) or at 60°C, but the reduction was greatest at 60°C. In the second experiment, a separate tissue suspension in saline was prepared from CNS tissue from each of 4 scrapie positive sheep and from each of 4 negative sheep. All specimens contained bacteria and after 90 days of incubation at 60°C, PrP Sc in CNS specimens was degraded beyond the detection limit in tissues from 2 scrapie positive sheep and was partially degraded in the other two specimens. The tissues from scrapie negative sheep were consistently negative for PrP Sc . Analysis of microbial 16S ribosomal DNA indicated that during the 90 day incubation period the microbe population shifted from a predominance of mesophiles to thermophiles, based on guanine-cytosine (GC) content of ribosomal RNA genes. The results in this study suggest that microbes commonly found in sheep carcasses or manure could play a role in the degradation of PrP Sc in CNS tissues during incubation at 60°C." @default.
- W2023642742 created "2016-06-24" @default.
- W2023642742 creator A5010827282 @default.
- W2023642742 creator A5048385143 @default.
- W2023642742 creator A5051278398 @default.
- W2023642742 date "2010-05-30" @default.
- W2023642742 modified "2023-09-26" @default.
- W2023642742 title "In Vitro Microbial Degradation of Abnormal Prions in Central Nervous System from Scrapie Affected Sheep" @default.
- W2023642742 cites W1487425252 @default.
- W2023642742 cites W1800445109 @default.
- W2023642742 cites W1840182669 @default.
- W2023642742 cites W1870615197 @default.
- W2023642742 cites W1976928436 @default.
- W2023642742 cites W1997935103 @default.
- W2023642742 cites W2016669691 @default.
- W2023642742 cites W2018790021 @default.
- W2023642742 cites W2035703126 @default.
- W2023642742 cites W2035961779 @default.
- W2023642742 cites W2038118251 @default.
- W2023642742 cites W2047251685 @default.
- W2023642742 cites W2048393965 @default.
- W2023642742 cites W2061259280 @default.
- W2023642742 cites W2080349754 @default.
- W2023642742 cites W2080811931 @default.
- W2023642742 cites W2087520649 @default.
- W2023642742 cites W2087873094 @default.
- W2023642742 cites W2108078938 @default.
- W2023642742 cites W2117034570 @default.
- W2023642742 cites W2121960537 @default.
- W2023642742 cites W2122007620 @default.
- W2023642742 cites W2126675843 @default.
- W2023642742 cites W2143207952 @default.
- W2023642742 cites W2163151366 @default.
- W2023642742 cites W2171710359 @default.
- W2023642742 cites W2221230083 @default.
- W2023642742 cites W2405853180 @default.
- W2023642742 cites W1519722302 @default.
- W2023642742 cites W43627389 @default.
- W2023642742 doi "https://doi.org/10.2174/1874318801004010020" @default.
- W2023642742 hasPublicationYear "2010" @default.
- W2023642742 type Work @default.
- W2023642742 sameAs 2023642742 @default.
- W2023642742 citedByCount "4" @default.
- W2023642742 countsByYear W20236427422013 @default.
- W2023642742 countsByYear W20236427422014 @default.
- W2023642742 countsByYear W20236427422015 @default.
- W2023642742 countsByYear W20236427422018 @default.
- W2023642742 crossrefType "journal-article" @default.
- W2023642742 hasAuthorship W2023642742A5010827282 @default.
- W2023642742 hasAuthorship W2023642742A5048385143 @default.
- W2023642742 hasAuthorship W2023642742A5051278398 @default.
- W2023642742 hasBestOaLocation W20236427421 @default.
- W2023642742 hasConcept C104317684 @default.
- W2023642742 hasConcept C142724271 @default.
- W2023642742 hasConcept C143701427 @default.
- W2023642742 hasConcept C144024400 @default.
- W2023642742 hasConcept C149923435 @default.
- W2023642742 hasConcept C180032290 @default.
- W2023642742 hasConcept C203014093 @default.
- W2023642742 hasConcept C25642318 @default.
- W2023642742 hasConcept C2776735649 @default.
- W2023642742 hasConcept C2779134260 @default.
- W2023642742 hasConcept C2908647359 @default.
- W2023642742 hasConcept C3019804061 @default.
- W2023642742 hasConcept C523546767 @default.
- W2023642742 hasConcept C54355233 @default.
- W2023642742 hasConcept C55493867 @default.
- W2023642742 hasConcept C67905577 @default.
- W2023642742 hasConcept C71924100 @default.
- W2023642742 hasConcept C86803240 @default.
- W2023642742 hasConcept C89423630 @default.
- W2023642742 hasConceptScore W2023642742C104317684 @default.
- W2023642742 hasConceptScore W2023642742C142724271 @default.
- W2023642742 hasConceptScore W2023642742C143701427 @default.
- W2023642742 hasConceptScore W2023642742C144024400 @default.
- W2023642742 hasConceptScore W2023642742C149923435 @default.
- W2023642742 hasConceptScore W2023642742C180032290 @default.
- W2023642742 hasConceptScore W2023642742C203014093 @default.
- W2023642742 hasConceptScore W2023642742C25642318 @default.
- W2023642742 hasConceptScore W2023642742C2776735649 @default.
- W2023642742 hasConceptScore W2023642742C2779134260 @default.
- W2023642742 hasConceptScore W2023642742C2908647359 @default.
- W2023642742 hasConceptScore W2023642742C3019804061 @default.
- W2023642742 hasConceptScore W2023642742C523546767 @default.
- W2023642742 hasConceptScore W2023642742C54355233 @default.
- W2023642742 hasConceptScore W2023642742C55493867 @default.
- W2023642742 hasConceptScore W2023642742C67905577 @default.
- W2023642742 hasConceptScore W2023642742C71924100 @default.
- W2023642742 hasConceptScore W2023642742C86803240 @default.
- W2023642742 hasConceptScore W2023642742C89423630 @default.
- W2023642742 hasLocation W20236427421 @default.
- W2023642742 hasOpenAccess W2023642742 @default.
- W2023642742 hasPrimaryLocation W20236427421 @default.
- W2023642742 hasRelatedWork W2091051652 @default.
- W2023642742 isParatext "false" @default.
- W2023642742 isRetracted "false" @default.
- W2023642742 magId "2023642742" @default.
- W2023642742 workType "article" @default.