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- W2023643333 abstract "The mouse embryonal stem cell line BLC 1 growing on feeder layer was treated with a calcium phosphate/DNA precipitate prepared with DNA of plasmid pAG60 which harbors the Tn5-derived neo gene, thus encoding resistance to G418, an aminoglycoside antibiotic. Transfection performed on feeder layer resulted in the formation of G418-resistant clones T1 and T2/K26. The stable integration of the transformed neo gene was confirmed by dot hybridization in all descendant cultures of clones T1 and T2/K26 as well as in the tumors derived from them. In vivo and in vitro differentiation revealed the pluripotent status of the transformants. Tumors derived from T1 and T2/K26 contained various tissues with derivatives of all three primary germ layers." @default.
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- W2023643333 date "1984-12-01" @default.
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- W2023643333 title "DNA transformation of a pluripotent mouse embryonal stem cell line with a dominant selective marker." @default.
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- W2023643333 doi "https://doi.org/10.1016/0045-6039(84)90058-7" @default.
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