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- W2023644088 abstract "Large volume injections —involving on-column focusing— were evaluated for packed micro and capillary liquid chromatography columns of 300 μm and 1.0 mm inner diameter (I.D.) respectively. It was found that the I.D. of the injection loop plays a critical role in sample dispersion, effecting peak asymmetry and injection reproductibility. The use of injection loops with too large or too small an I.D. resulted in a reduced injection performance. Ideally the I.D. should be in between 100–150 μm. Further it was investigated what influence the loop volume and the use of a low dispersion injection technique had on the column stability. The combination of large volume injections—up to 1 μl and 5 μl for 300 μm and 1.0 mm I.D. columns, respectively—and additional switching of the injection valve did not affect column lifetime. Typical decreases of only 10% in efficiency over 1500 injections were found." @default.
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- W2023644088 date "1996-10-01" @default.
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- W2023644088 title "Optimised injection techniques for micro and capillary liquid chromatography" @default.
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