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- W2023644422 abstract "A diffusion cell and a polysulphone membrane were used for the estimation of the effective diffusion coefficient (DM) of endoglucanase and lysozyme. DM was calculated from the slope obtained by fitting the amount of protein transferred through the membrane against time using the appropriate mathematical equation for the steady state. Different protein concentrations and pH values were tested. The calculated DM for endoglucanase was in the order of 10−13 m2 s−1 whereas the calculated DM for lysozyme was an order of magnitude lower. A diffusion coefficient dependence on the concentration and pH was observed. In addition, the partition coefficient, KM, of the two proteins between the membrane and a solution containing the proteins was calculated." @default.
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- W2023644422 date "1999-06-01" @default.
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- W2023644422 title "Estimation of endoglucanase and lysozyme effective diffusion coefficients in polysulphone membranes" @default.
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- W2023644422 doi "https://doi.org/10.1016/s0168-1656(99)00095-4" @default.
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