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- W2023646215 abstract "A dynamic pH gradient extends the separation power of zone electrophoresis to mixtures of substances with widely differing pK values. A simple method for the generation of dynamic changes in the pH of the background electrolyte in the separation capillary is described. It is based on a controlled modification of the composition and, thus, of the pH of an electrolyte in the electrode chamber at the injection end of the separation capillary during the analysis. The higher the concentration of H+ in this electrode chamber, the higher is the electromigration flow of H+ into the capillary and the lower is the pH of the actual background electrolyte therein. The utilization of this principle is exemplified by the separation of eleven purine and pyrimidine bases with pK values ranging from 6.0 to 1.9." @default.
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- W2023646215 date "1989-01-01" @default.
- W2023646215 modified "2023-10-16" @default.
- W2023646215 title "Simple method for generation of dymanic pH gradient in capillary zone electrophoresis" @default.
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- W2023646215 doi "https://doi.org/10.1016/s0021-9673(01)84294-1" @default.
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