FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023652532> ?p ?o ?g. }

• W2023652532 endingPage "238" @default.
• W2023652532 startingPage "232" @default.
• W2023652532 abstract "We assessed the validity of monitoring changes in mitochondrial membrane potential (ΔΨ) with a fluorescent probe, JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl benzimidazolo-carbocyanine iodide), for the quantitative evaluation of neonatal hypoxic-ischemic brain injury. Seven-day-old rat pups were subjected to 2h of 8% oxygen following unilateral carotid artery ligation. Brain tissue was obtained for JC-1 staining at 24h after hypoxia ischemia (HI), and the results were compared with those of other simultaneous measurements such as flow cytometry with fluoresceinated annexin V/propidium iodide (PI), terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling (TUNEL) staining, triphenyl tetrazolium chloride (TTC) infarct area and western blot for cytosolic cytochrome c. Flow cytograms of JC-1 showed two distinct sub-populations with different ΔΨ, red with high ΔΨ and green with low ΔΨ, at 24h after HI. This shift of JC-1 fluorescence from red to green indicated a collapse of ΔΨ. The increased percentage of low ΔΨ with JC-1 showed a significant positive correlation with a simultaneous increase in annexin V(+)/PI(+) necrotic cells, TUNEL-positive cells, TTC infarct area and western blot of cytosolic cytochrome c, and negative correlation with annexin V(-)/PI(-) live cells. In summary, low ΔΨ measured with JC-1 was significantly correlated with results from other methods used to assess the extent of brain damage after HI. Therefore, fluorocytometric analysis of ΔΨ with JC-1 might be a sensitive and reliable technique in the quantitative evaluation of neonatal brain injury." @default.
• W2023652532 created "2016-06-24" @default.
• W2023652532 creator A5018338653 @default.
• W2023652532 creator A5019852528 @default.
• W2023652532 creator A5048288085 @default.
• W2023652532 creator A5052781438 @default.
• W2023652532 creator A5065648607 @default.
• W2023652532 creator A5080492645 @default.
• W2023652532 date "2010-11-01" @default.
• W2023652532 modified "2023-09-24" @default.
• W2023652532 title "Comparative evaluation of hypoxic–ischemic brain injury by flow cytometric analysis of mitochondrial membrane potential with JC-1 in neonatal rats" @default.
• W2023652532 cites W1154178244 @default.
• W2023652532 cites W1566271574 @default.
• W2023652532 cites W1966539065 @default.
• W2023652532 cites W1996568613 @default.
• W2023652532 cites W2015101141 @default.
• W2023652532 cites W2022523356 @default.
• W2023652532 cites W2036937264 @default.
• W2023652532 cites W2044745267 @default.
• W2023652532 cites W2053999496 @default.
• W2023652532 cites W2061354386 @default.
• W2023652532 cites W2070854437 @default.
• W2023652532 cites W2072858020 @default.
• W2023652532 cites W2092146469 @default.
• W2023652532 cites W2092441665 @default.
• W2023652532 cites W2109386698 @default.
• W2023652532 cites W2112696881 @default.
• W2023652532 cites W2138302361 @default.
• W2023652532 doi "https://doi.org/10.1016/j.jneumeth.2010.08.020" @default.
• W2023652532 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20817028" @default.
• W2023652532 hasPublicationYear "2010" @default.
• W2023652532 type Work @default.
• W2023652532 sameAs 2023652532 @default.
• W2023652532 citedByCount "29" @default.
• W2023652532 countsByYear W20236525322012 @default.
• W2023652532 countsByYear W20236525322013 @default.
• W2023652532 countsByYear W20236525322014 @default.
• W2023652532 countsByYear W20236525322015 @default.
• W2023652532 countsByYear W20236525322016 @default.
• W2023652532 countsByYear W20236525322017 @default.
• W2023652532 countsByYear W20236525322018 @default.
• W2023652532 countsByYear W20236525322019 @default.
• W2023652532 countsByYear W20236525322020 @default.
• W2023652532 countsByYear W20236525322021 @default.
• W2023652532 countsByYear W20236525322022 @default.
• W2023652532 countsByYear W20236525322023 @default.
• W2023652532 crossrefType "journal-article" @default.
• W2023652532 hasAuthorship W2023652532A5018338653 @default.
• W2023652532 hasAuthorship W2023652532A5019852528 @default.
• W2023652532 hasAuthorship W2023652532A5048288085 @default.
• W2023652532 hasAuthorship W2023652532A5052781438 @default.
• W2023652532 hasAuthorship W2023652532A5065648607 @default.
• W2023652532 hasAuthorship W2023652532A5080492645 @default.
• W2023652532 hasConcept C104317684 @default.
• W2023652532 hasConcept C126322002 @default.
• W2023652532 hasConcept C142724271 @default.
• W2023652532 hasConcept C153911025 @default.
• W2023652532 hasConcept C185592680 @default.
• W2023652532 hasConcept C190283241 @default.
• W2023652532 hasConcept C196795494 @default.
• W2023652532 hasConcept C2775934118 @default.
• W2023652532 hasConcept C2776390042 @default.
• W2023652532 hasConcept C2776415932 @default.
• W2023652532 hasConcept C2781458332 @default.
• W2023652532 hasConcept C29311851 @default.
• W2023652532 hasConcept C31573885 @default.
• W2023652532 hasConcept C541997718 @default.
• W2023652532 hasConcept C553184892 @default.
• W2023652532 hasConcept C55493867 @default.
• W2023652532 hasConcept C71924100 @default.
• W2023652532 hasConcept C74864618 @default.
• W2023652532 hasConcept C86803240 @default.
• W2023652532 hasConcept C88634738 @default.
• W2023652532 hasConceptScore W2023652532C104317684 @default.
• W2023652532 hasConceptScore W2023652532C126322002 @default.
• W2023652532 hasConceptScore W2023652532C142724271 @default.
• W2023652532 hasConceptScore W2023652532C153911025 @default.
• W2023652532 hasConceptScore W2023652532C185592680 @default.
• W2023652532 hasConceptScore W2023652532C190283241 @default.
• W2023652532 hasConceptScore W2023652532C196795494 @default.
• W2023652532 hasConceptScore W2023652532C2775934118 @default.
• W2023652532 hasConceptScore W2023652532C2776390042 @default.
• W2023652532 hasConceptScore W2023652532C2776415932 @default.
• W2023652532 hasConceptScore W2023652532C2781458332 @default.
• W2023652532 hasConceptScore W2023652532C29311851 @default.
• W2023652532 hasConceptScore W2023652532C31573885 @default.
• W2023652532 hasConceptScore W2023652532C541997718 @default.
• W2023652532 hasConceptScore W2023652532C553184892 @default.
• W2023652532 hasConceptScore W2023652532C55493867 @default.
• W2023652532 hasConceptScore W2023652532C71924100 @default.
• W2023652532 hasConceptScore W2023652532C74864618 @default.
• W2023652532 hasConceptScore W2023652532C86803240 @default.
• W2023652532 hasConceptScore W2023652532C88634738 @default.
• W2023652532 hasIssue "2" @default.
• W2023652532 hasLocation W20236525321 @default.
• W2023652532 hasLocation W20236525322 @default.
• W2023652532 hasOpenAccess W2023652532 @default.
• W2023652532 hasPrimaryLocation W20236525321 @default.

• next