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• W2023653707 abstract "The objective of the present study was to develop a new method for the simultaneous quantitation of imperatorin and its metabolite xanthotoxol in rat plasma. The samples were prepared with hollow fiber liquid phase microextraction (HF-LPME). The optimized extraction procedure was acquired by assessing extraction solvent, length of the fiber, agitation rate, extraction temperature and time. A comparison of sample pretreatment ways between HF-LPME and deproteinization with methanol was performed, which demonstrated less ion suppression and better sensitivity of HF-LPME. Analytes were separated on a C18 column with a gradient elution consisted of methanol and water containing 1mmol/L ammonium acetate. The detection was accomplished by electrospray ionization (ESI) source operating in the positive ionization mode. Selected-multiple-reaction monitoring (SMRM) scanning was employed, which guaranteed a higher sensitivity compared with MRM mode. Calibration curves were linear over investigated ranges with correlation coefficients greater than 0.9979. Precision varied from 0.26% to 14%, and the accuracy varied within ±5.5%. The developed method was successfully applied to the pharmacokinetic research of imperatorin and its metabolite xanthotoxol after oral administration of imperatorin to rats." @default.
• W2023653707 created "2016-06-24" @default.
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• W2023653707 date "2014-01-01" @default.
• W2023653707 modified "2023-09-24" @default.
• W2023653707 title "Simultaneous determination of imperatorin and its metabolite xanthotoxol in rat plasma by using HPLC–ESI-MS coupled with hollow fiber liquid phase microextraction" @default.
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• W2023653707 doi "https://doi.org/10.1016/j.jchromb.2013.11.050" @default.
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