FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023676229> ?p ?o ?g. }

• W2023676229 endingPage "2923" @default.
• W2023676229 startingPage "2909" @default.
• W2023676229 abstract "Apo-Calmodulin acts as the light chain for unconventional myosin V, and treatment with Ca(2+) can cause dissociation of calmodulin from the 6IQ region of the myosin heavy chain. The effects of Ca(2+) on the stoichiometry and affinity of interactions of calmodulin and its two domains with two myosin-V peptides (IQ3 and IQ4) have therefore been quantified in vitro, using fluorescence and near- and far-UV CD. The results with separate domains show their differential affinity in interactions with the IQ motif, with the apo-N domain interacting surprisingly weakly. Contrary to expectations, the effect of Ca(2+) on the interactions of either peptide with either isolated domain is to increase affinity, reducing the K(d) at physiological ionic strengths by >200-fold to approximately 75 nM for the N domain, and approximately 10-fold to approximately 15 nM for the C domain. Under suitable conditions, intact (holo- or apo-) calmodulin can bind up to two IQ-target sequences. Interactions of apo- and holo-calmodulin with the double-length, concatenated sequence (IQ34) can result in complex stoichiometries. Strikingly, holo-calmodulin forms a high-affinity 1:1 complex with IQ34 in a novel mode of interaction, as a bridged structure wherein two calmodulin domains interact with adjacent IQ motifs. This apparently imposes a steric requirement for the alpha-helical target sequence to be discontinuous, possibly in the central region, and a model structure is illustrated. Such a mode of interaction could account for the Ca(2+)-dependent regulation of myosin V in vitro motility, by changing the structure of the regulatory complex, and paradoxically causing calmodulin dissociation through a change in stoichiometry, rather than a Ca(2+)-dependent reduction in affinity." @default.
• W2023676229 created "2016-06-24" @default.
• W2023676229 creator A5038418557 @default.
• W2023676229 creator A5043170875 @default.
• W2023676229 date "2002-12-01" @default.
• W2023676229 modified "2023-10-10" @default.
• W2023676229 title "Regulatory implications of a novel mode of interaction of calmodulin with a double IQ-motif target sequence from murine dilute myosin V" @default.
• W2023676229 cites W1486466859 @default.
• W2023676229 cites W1514691814 @default.
• W2023676229 cites W1518396254 @default.
• W2023676229 cites W1520978489 @default.
• W2023676229 cites W1550790333 @default.
• W2023676229 cites W1565248232 @default.
• W2023676229 cites W1568116533 @default.
• W2023676229 cites W1576403707 @default.
• W2023676229 cites W1576751611 @default.
• W2023676229 cites W1592692300 @default.
• W2023676229 cites W1599277155 @default.
• W2023676229 cites W1601913333 @default.
• W2023676229 cites W1964723385 @default.
• W2023676229 cites W1965661118 @default.
• W2023676229 cites W1967305385 @default.
• W2023676229 cites W1971584686 @default.
• W2023676229 cites W1972237784 @default.
• W2023676229 cites W1973838961 @default.
• W2023676229 cites W1982252438 @default.
• W2023676229 cites W1982554911 @default.
• W2023676229 cites W1990110770 @default.
• W2023676229 cites W2015426350 @default.
• W2023676229 cites W2015642465 @default.
• W2023676229 cites W2017124830 @default.
• W2023676229 cites W2022332311 @default.
• W2023676229 cites W2024398910 @default.
• W2023676229 cites W2025251010 @default.
• W2023676229 cites W2025853178 @default.
• W2023676229 cites W2029870632 @default.
• W2023676229 cites W2030890665 @default.
• W2023676229 cites W2031001454 @default.
• W2023676229 cites W2031811477 @default.
• W2023676229 cites W2034454731 @default.
• W2023676229 cites W2037668423 @default.
• W2023676229 cites W2052366943 @default.
• W2023676229 cites W2053330127 @default.
• W2023676229 cites W2063963320 @default.
• W2023676229 cites W2064638216 @default.
• W2023676229 cites W2067394407 @default.
• W2023676229 cites W2083247866 @default.
• W2023676229 cites W2087506595 @default.
• W2023676229 cites W2096942328 @default.
• W2023676229 cites W2104017463 @default.
• W2023676229 cites W2104018418 @default.
• W2023676229 cites W2106940418 @default.
• W2023676229 cites W2120385383 @default.
• W2023676229 cites W2126181422 @default.
• W2023676229 cites W2126551164 @default.
• W2023676229 cites W2131750624 @default.
• W2023676229 cites W2139183106 @default.
• W2023676229 cites W2159497398 @default.
• W2023676229 cites W2168398034 @default.
• W2023676229 cites W2172712627 @default.
• W2023676229 cites W4229913877 @default.
• W2023676229 doi "https://doi.org/10.1110/ps.0210402" @default.
• W2023676229 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2373755" @default.
• W2023676229 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12441389" @default.
• W2023676229 hasPublicationYear "2002" @default.
• W2023676229 type Work @default.
• W2023676229 sameAs 2023676229 @default.
• W2023676229 citedByCount "37" @default.
• W2023676229 countsByYear W20236762292013 @default.
• W2023676229 countsByYear W20236762292014 @default.
• W2023676229 countsByYear W20236762292016 @default.
• W2023676229 countsByYear W20236762292017 @default.
• W2023676229 countsByYear W20236762292023 @default.
• W2023676229 crossrefType "journal-article" @default.
• W2023676229 hasAuthorship W2023676229A5038418557 @default.
• W2023676229 hasAuthorship W2023676229A5043170875 @default.
• W2023676229 hasBestOaLocation W20236762292 @default.
• W2023676229 hasConcept C12554922 @default.
• W2023676229 hasConcept C181199279 @default.
• W2023676229 hasConcept C185592680 @default.
• W2023676229 hasConcept C29688787 @default.
• W2023676229 hasConcept C51639874 @default.
• W2023676229 hasConcept C55493867 @default.
• W2023676229 hasConcept C6997183 @default.
• W2023676229 hasConcept C8035138 @default.
• W2023676229 hasConcept C86803240 @default.
• W2023676229 hasConceptScore W2023676229C12554922 @default.
• W2023676229 hasConceptScore W2023676229C181199279 @default.
• W2023676229 hasConceptScore W2023676229C185592680 @default.
• W2023676229 hasConceptScore W2023676229C29688787 @default.
• W2023676229 hasConceptScore W2023676229C51639874 @default.
• W2023676229 hasConceptScore W2023676229C55493867 @default.
• W2023676229 hasConceptScore W2023676229C6997183 @default.
• W2023676229 hasConceptScore W2023676229C8035138 @default.
• W2023676229 hasConceptScore W2023676229C86803240 @default.
• W2023676229 hasIssue "12" @default.
• W2023676229 hasLocation W20236762291 @default.
• W2023676229 hasLocation W20236762292 @default.

• next