FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023677697> ?p ?o ?g. }

• W2023677697 abstract "In order to understand the mechanism of oocyte maturation in seasonal-breeding wild frogs, we have cloned and sequenced a cDNA encoding Cdc2 kinase, a component of the maturation-promoting factor (MPF) in Rana dybowskii. About 1.2-kb cDNA was isolated by reverse transcription coupled to polymerase chain reaction (RT-PCR) and cDNA library screening. The cloned Rana Cdc2 cDNA encodes a complete open-reading frame with 302 amino acid residues, which deduce a 34-kDa protein. Homology of more than 80% was found between the deduced amino acid sequence of Rana Cdc2 and that of five phylogenetically distant organisms, and 94% identity was found between Rana and Xenopus. More importantly, the Thr14, Tyr15, and Thr161 residues, the phosphorylation sites for the activation of the enzyme, are highly conserved. In vitro-translated Rana Cdc2 cross-reacted with Xenopus p34(cdc2) antibody as shown by Western blot. Northern blot analysis showed that a 1.7-kb transcript was highly expressed in the gonads compared to other tissues, indicating the important role of Cdc2 kinase in gonads as a component of MPF. The cloned Rana Cdc2 cDNA also exhibited histone H1 kinase activity when expressed in CV-1 cells. In the present study, therefore, we have characterized the Rana Cdc2 kinase in amphibian, which will be helpful in understanding the process of oocyte maturation related to the reproduction cycle of wild frogs." @default.
• W2023677697 created "2016-06-24" @default.
• W2023677697 creator A5019470350 @default.
• W2023677697 creator A5055350629 @default.
• W2023677697 creator A5060664681 @default.
• W2023677697 creator A5075781286 @default.
• W2023677697 creator A5080674043 @default.
• W2023677697 date "2000-02-01" @default.
• W2023677697 modified "2023-09-27" @default.
• W2023677697 title "Cloning and Characterization of cDNA Encoding Cdc2 Kinase, a Component of Maturation-Promoting Factor, in Rana dybowskii" @default.
• W2023677697 cites W1494766755 @default.
• W2023677697 cites W1723327061 @default.
• W2023677697 cites W1982752345 @default.
• W2023677697 cites W1989244997 @default.
• W2023677697 cites W2003770359 @default.
• W2023677697 cites W2010414017 @default.
• W2023677697 cites W2011802485 @default.
• W2023677697 cites W2013549179 @default.
• W2023677697 cites W2020257412 @default.
• W2023677697 cites W2026466385 @default.
• W2023677697 cites W2031086068 @default.
• W2023677697 cites W2031576542 @default.
• W2023677697 cites W2032413267 @default.
• W2023677697 cites W2034051609 @default.
• W2023677697 cites W2037810181 @default.
• W2023677697 cites W2047799300 @default.
• W2023677697 cites W2050341582 @default.
• W2023677697 cites W2051657790 @default.
• W2023677697 cites W2054760302 @default.
• W2023677697 cites W2057811505 @default.
• W2023677697 cites W2059674135 @default.
• W2023677697 cites W2070229091 @default.
• W2023677697 cites W2072720547 @default.
• W2023677697 cites W2102242413 @default.
• W2023677697 cites W2117939247 @default.
• W2023677697 cites W2127283099 @default.
• W2023677697 cites W2134812217 @default.
• W2023677697 cites W2144634347 @default.
• W2023677697 cites W2154128645 @default.
• W2023677697 cites W2471666453 @default.
• W2023677697 cites W247355068 @default.
• W2023677697 doi "https://doi.org/10.1006/gcen.1999.7420" @default.
• W2023677697 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10642452" @default.
• W2023677697 hasPublicationYear "2000" @default.
• W2023677697 type Work @default.
• W2023677697 sameAs 2023677697 @default.
• W2023677697 citedByCount "4" @default.
• W2023677697 countsByYear W20236776972023 @default.
• W2023677697 crossrefType "journal-article" @default.
• W2023677697 hasAuthorship W2023677697A5019470350 @default.
• W2023677697 hasAuthorship W2023677697A5055350629 @default.
• W2023677697 hasAuthorship W2023677697A5060664681 @default.
• W2023677697 hasAuthorship W2023677697A5075781286 @default.
• W2023677697 hasAuthorship W2023677697A5080674043 @default.
• W2023677697 hasConcept C104317684 @default.
• W2023677697 hasConcept C120504264 @default.
• W2023677697 hasConcept C153911025 @default.
• W2023677697 hasConcept C177707886 @default.
• W2023677697 hasConcept C187882448 @default.
• W2023677697 hasConcept C189482833 @default.
• W2023677697 hasConcept C19924922 @default.
• W2023677697 hasConcept C2779535977 @default.
• W2023677697 hasConcept C29537977 @default.
• W2023677697 hasConcept C50724178 @default.
• W2023677697 hasConcept C54355233 @default.
• W2023677697 hasConcept C86803240 @default.
• W2023677697 hasConceptScore W2023677697C104317684 @default.
• W2023677697 hasConceptScore W2023677697C120504264 @default.
• W2023677697 hasConceptScore W2023677697C153911025 @default.
• W2023677697 hasConceptScore W2023677697C177707886 @default.
• W2023677697 hasConceptScore W2023677697C187882448 @default.
• W2023677697 hasConceptScore W2023677697C189482833 @default.
• W2023677697 hasConceptScore W2023677697C19924922 @default.
• W2023677697 hasConceptScore W2023677697C2779535977 @default.
• W2023677697 hasConceptScore W2023677697C29537977 @default.
• W2023677697 hasConceptScore W2023677697C50724178 @default.
• W2023677697 hasConceptScore W2023677697C54355233 @default.
• W2023677697 hasConceptScore W2023677697C86803240 @default.
• W2023677697 hasLocation W20236776971 @default.
• W2023677697 hasLocation W20236776972 @default.
• W2023677697 hasOpenAccess W2023677697 @default.
• W2023677697 hasPrimaryLocation W20236776971 @default.
• W2023677697 hasRelatedWork W1965489627 @default.
• W2023677697 hasRelatedWork W198344517 @default.
• W2023677697 hasRelatedWork W2003723740 @default.
• W2023677697 hasRelatedWork W2007117365 @default.
• W2023677697 hasRelatedWork W2009337267 @default.
• W2023677697 hasRelatedWork W2016201353 @default.
• W2023677697 hasRelatedWork W2024361802 @default.
• W2023677697 hasRelatedWork W2034835431 @default.
• W2023677697 hasRelatedWork W2040382281 @default.
• W2023677697 hasRelatedWork W2042149904 @default.
• W2023677697 hasRelatedWork W2046256904 @default.
• W2023677697 hasRelatedWork W2049221108 @default.
• W2023677697 hasRelatedWork W2060560178 @default.
• W2023677697 hasRelatedWork W2061401401 @default.
• W2023677697 hasRelatedWork W2072640701 @default.
• W2023677697 hasRelatedWork W2075083036 @default.
• W2023677697 hasRelatedWork W2102718428 @default.
• W2023677697 hasRelatedWork W2112847380 @default.

• next