Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023679747> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2023679747 endingPage "30" @default.
- W2023679747 startingPage "25" @default.
- W2023679747 abstract "Previously, in order to evaluate the migration of specific types of lymphocytes (e.g., T or B cells, CD4 or CD8 subsets) using the Boyden chamber technique, the relevant cell populations have first been purified. We report here a method which permits identification of the lymphocytes following migration into the nitrocellulose filters. After fixation, the filters are exposed to specific anti-lymphocyte monoclonal antibodies and the reaction is visualized via a second gold-linked antibody. Monocyte-depleted lymphocytes isolated from human peripheral blood are routinely used for migration but mixed mononuclear cell preparations can also be used. This was shown by comparing lymphocyte migration in monocyte-containing and monocyte-depleted cell suspensions isolated from the same donor. The presence of monocytes did not influence the migration of normal resting T lymphocytes. With human peripheral blood as starting material the method is most suitable for the evaluation of T cell migration since, in most instances, T cells are the predominant lymphocyte population. When the B cell count is normal (5-10% total lymphocytes), quantification of B cell migration requires enrichment but not complete purification of this population. Migrating monocytes can also be identified. However, the antibody staining is less intense on the spread monocytes and, therefore, quantification must be performed by eye rather than with the Optomax image analyser." @default.
- W2023679747 created "2016-06-24" @default.
- W2023679747 creator A5003056562 @default.
- W2023679747 creator A5034001153 @default.
- W2023679747 creator A5039338620 @default.
- W2023679747 creator A5044352811 @default.
- W2023679747 creator A5080714451 @default.
- W2023679747 date "1991-05-01" @default.
- W2023679747 modified "2023-09-24" @default.
- W2023679747 title "Identification and quantification of human peripheral blood lymphocytes and monocytes following migration into nitrocellulose filters" @default.
- W2023679747 cites W1017844775 @default.
- W2023679747 cites W1503025822 @default.
- W2023679747 cites W2058965205 @default.
- W2023679747 cites W2084263070 @default.
- W2023679747 cites W2113245275 @default.
- W2023679747 cites W2127036529 @default.
- W2023679747 cites W2147294308 @default.
- W2023679747 cites W2282054059 @default.
- W2023679747 cites W2439981771 @default.
- W2023679747 cites W944167413 @default.
- W2023679747 doi "https://doi.org/10.1016/0022-1759(91)90347-i" @default.
- W2023679747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2040815" @default.
- W2023679747 hasPublicationYear "1991" @default.
- W2023679747 type Work @default.
- W2023679747 sameAs 2023679747 @default.
- W2023679747 citedByCount "0" @default.
- W2023679747 crossrefType "journal-article" @default.
- W2023679747 hasAuthorship W2023679747A5003056562 @default.
- W2023679747 hasAuthorship W2023679747A5034001153 @default.
- W2023679747 hasAuthorship W2023679747A5039338620 @default.
- W2023679747 hasAuthorship W2023679747A5044352811 @default.
- W2023679747 hasAuthorship W2023679747A5080714451 @default.
- W2023679747 hasConcept C137061746 @default.
- W2023679747 hasConcept C1491633281 @default.
- W2023679747 hasConcept C153911025 @default.
- W2023679747 hasConcept C159654299 @default.
- W2023679747 hasConcept C202751555 @default.
- W2023679747 hasConcept C203014093 @default.
- W2023679747 hasConcept C2777761686 @default.
- W2023679747 hasConcept C2781184567 @default.
- W2023679747 hasConcept C2908647359 @default.
- W2023679747 hasConcept C542903549 @default.
- W2023679747 hasConcept C55493867 @default.
- W2023679747 hasConcept C71924100 @default.
- W2023679747 hasConcept C86803240 @default.
- W2023679747 hasConcept C99454951 @default.
- W2023679747 hasConceptScore W2023679747C137061746 @default.
- W2023679747 hasConceptScore W2023679747C1491633281 @default.
- W2023679747 hasConceptScore W2023679747C153911025 @default.
- W2023679747 hasConceptScore W2023679747C159654299 @default.
- W2023679747 hasConceptScore W2023679747C202751555 @default.
- W2023679747 hasConceptScore W2023679747C203014093 @default.
- W2023679747 hasConceptScore W2023679747C2777761686 @default.
- W2023679747 hasConceptScore W2023679747C2781184567 @default.
- W2023679747 hasConceptScore W2023679747C2908647359 @default.
- W2023679747 hasConceptScore W2023679747C542903549 @default.
- W2023679747 hasConceptScore W2023679747C55493867 @default.
- W2023679747 hasConceptScore W2023679747C71924100 @default.
- W2023679747 hasConceptScore W2023679747C86803240 @default.
- W2023679747 hasConceptScore W2023679747C99454951 @default.
- W2023679747 hasIssue "1" @default.
- W2023679747 hasLocation W20236797471 @default.
- W2023679747 hasLocation W20236797472 @default.
- W2023679747 hasOpenAccess W2023679747 @default.
- W2023679747 hasPrimaryLocation W20236797471 @default.
- W2023679747 hasRelatedWork W1567474693 @default.
- W2023679747 hasRelatedWork W1973692620 @default.
- W2023679747 hasRelatedWork W1991644080 @default.
- W2023679747 hasRelatedWork W2030611884 @default.
- W2023679747 hasRelatedWork W2041064855 @default.
- W2023679747 hasRelatedWork W2063428357 @default.
- W2023679747 hasRelatedWork W2102639438 @default.
- W2023679747 hasRelatedWork W2368460920 @default.
- W2023679747 hasRelatedWork W2380360044 @default.
- W2023679747 hasRelatedWork W2406657306 @default.
- W2023679747 hasVolume "139" @default.
- W2023679747 isParatext "false" @default.
- W2023679747 isRetracted "false" @default.
- W2023679747 magId "2023679747" @default.
- W2023679747 workType "article" @default.