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- W2023681892 abstract "Abstract BACKGROUND: Polyphenol oxidase (PPO) causes the postharvest loss of fruits and vegetables but is also a key factor in the quality development of tea. However, there are no reports on engineered active plant PPO purified from prokaryotic cells. RESULTS: In this study the ppo gene of about 1800 bp from Camellia sinensis cv. Yihongzao was successfully cloned and expressed in Escherichia coli . The PPOs purified from both the soluble fraction and the inclusion bodies showed activity. In addition, 1.0 × 10 −7 mol L −1 Cu 2+ and acidic conditions were found to be favourable for the engineered PPO catalysis of catechol oxidation. CONCLUSION: This paper represents the first report on C. sinensis ppo expression in E. coli and engineered active PPO purification. The results of the study provide a basis for the large‐scale preparation and application of PPO. Copyright © 2010 Society of Chemical Industry" @default.
- W2023681892 created "2016-06-24" @default.
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- W2023681892 date "2010-07-26" @default.
- W2023681892 modified "2023-10-12" @default.
- W2023681892 title "Prokaryotic expression and purification of <i>Camellia sinensis</i> polyphenol oxidase" @default.
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- W2023681892 doi "https://doi.org/10.1002/jsfa.4111" @default.
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