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- W2023682541 abstract "Abstract We have used Tl-oligonucleotide mapping to explore the nucleotide sequence organization of the Sendai virus genome. Duplexes of radioactive virus genes and unlabeled virus messenger RNA species, obtained by ribonuclease digestion of RNA hybrids, were separated by agarose gel electrophoresis. Identities of duplexes were deduced from their apparent molecular weights, relative to the molecular weights of the virus gene products. Five duplexes were assigned to the five genes that map at the 3′ side of the virus genome. However, oligonucleotide mapping revealed that there was not a simple relationship between genes and duplexes: (a) the 3′-terminal gene, NP, was present in two duplexes, the larger of which contained an additional oligonucleotide that may represent the template for the viral leader RNA; (b) the largest band in the group yielded more than twice as many oligonucleotides as any of the other duplexes and appears to represent a pair of duplexes containing the P and F 0 genes. The characteristic oligonucleotides of the L gene were identified separately; their abundance relative to the molecular weight of this gene suggested that the 5′ side of the viral genome is guanine-poor, compared to the 3′ side. Oligonucleotides of the full-length complement of the viral genome (50 S plus-strand) and of positive-sense virus messenger RNAs were also identified. These data provide a foundation for further structural analysis of the genome of this model paramyxovirus and its defective-interfering (DI) deletion mutants." @default.
- W2023682541 created "2016-06-24" @default.
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- W2023682541 date "1982-04-01" @default.
- W2023682541 modified "2023-09-26" @default.
- W2023682541 title "Sendai virus gene sequences identified by oligonucleotide mapping" @default.
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- W2023682541 doi "https://doi.org/10.1016/0042-6822(82)90314-2" @default.
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