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- W2023683208 abstract "The in vitro selection and simultaneous evolution of proteins is feasible by means of ribosome display. Here, we describe the use of a protein bearing a binding property without being an antibody for affinity enrichment of the ternary complex, consisting of a protein, a ribosome and a encoding mRNA. The binding property was a simple affinity tag, namely Strep-tag II and His-tag. We could demonstrate that the selection of a specific mRNA encoding a shortened bovine heart fatty acid binding protein with a N-terminal His-tag was possible. After nine cycles of transcription, translation, affinity selection and reverse transcription PCR the protein with the His-tag could be enriched 10(8)-fold." @default.
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- W2023683208 date "2001-07-24" @default.
- W2023683208 modified "2023-09-25" @default.
- W2023683208 title "In vitro selection of other proteins than antibodies by means of ribosome display" @default.
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- W2023683208 doi "https://doi.org/10.1016/s0014-5793(01)02660-6" @default.
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