FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023683758> ?p ?o ?g. }

• W2023683758 endingPage "1337" @default.
• W2023683758 startingPage "1329" @default.
• W2023683758 abstract "Background: The ycaC gene comprises a 621 base pair open reading frame in Escherichia coli. The ycaC gene product (ycaCgp) is uncharacterized and has no assigned function. The closest sequence homologs with an assigned function belong to a family of bacterial hydrolases that catalyze isochorismatase-like reactions, but these have only low sequence similarity to ycaCgp (∼20% amino acid identity). The ycaCgp was obtained and identified during crystallization trials of an unrelated E. coli protein with which it co-purified.Results: The 1.8 å crystal structure of ycaCgp reveals an octameric complex comprised of two tetrameric rings. A large three-layer (αβα) sandwich domain and a small helical domain form the folded structure of the monomeric unit. Comparisons with sequence and structure databases suggest that ycaCgp belongs to a diverse family of bacterial hydrolases. The most closely related three-dimensional structure is that of the D2 tetrameric N-carbamoylsarcosine amidohydrolase (CSHase) from an Arthrobacter species. A conspicuous cleft between two ycaCgp subunits contains several conserved residues including Cys118, which we propose to be catalytic. In the active site, a nonprolyl cis peptide bond precedes Val114 and coincides with a cis peptide bond in CSHase in a region of dissimilar sequence. The crystal structure reveals a probable error or mutation relative to the reported genomic sequence.Conclusions: Although the specific function of ycaCgp is not yet known, structural studies solidify the relationship of this protein to other hydrolases and illuminate its active site and key elements of the catalytic mechanism." @default.
• W2023683758 created "2016-06-24" @default.
• W2023683758 creator A5007964042 @default.
• W2023683758 creator A5050678734 @default.
• W2023683758 creator A5061217668 @default.
• W2023683758 date "1998-10-01" @default.
• W2023683758 modified "2023-09-23" @default.
• W2023683758 title "The 1.8 å crystal structure of the ycaC gene product from Escherichia coli reveals an octameric hydrolase of unknown specificity" @default.
• W2023683758 cites W1539796472 @default.
• W2023683758 cites W1584704387 @default.
• W2023683758 cites W1603300564 @default.
• W2023683758 cites W1608218663 @default.
• W2023683758 cites W1625574816 @default.
• W2023683758 cites W1926303070 @default.
• W2023683758 cites W1947962930 @default.
• W2023683758 cites W1971403296 @default.
• W2023683758 cites W1973795201 @default.
• W2023683758 cites W1977724110 @default.
• W2023683758 cites W1993321575 @default.
• W2023683758 cites W2001641653 @default.
• W2023683758 cites W2001882067 @default.
• W2023683758 cites W2002195659 @default.
• W2023683758 cites W2015292449 @default.
• W2023683758 cites W2017727500 @default.
• W2023683758 cites W2026640159 @default.
• W2023683758 cites W2028231353 @default.
• W2023683758 cites W2041652393 @default.
• W2023683758 cites W2043972921 @default.
• W2023683758 cites W2067316723 @default.
• W2023683758 cites W2071486470 @default.
• W2023683758 cites W2074231493 @default.
• W2023683758 cites W2078248419 @default.
• W2023683758 cites W2088933990 @default.
• W2023683758 cites W2100882223 @default.
• W2023683758 cites W2102993309 @default.
• W2023683758 cites W2106882534 @default.
• W2023683758 cites W2110132014 @default.
• W2023683758 cites W2134153749 @default.
• W2023683758 cites W2147694156 @default.
• W2023683758 cites W2157165549 @default.
• W2023683758 cites W2158714788 @default.
• W2023683758 cites W4239833500 @default.
• W2023683758 cites W4246356731 @default.
• W2023683758 cites W4295127969 @default.
• W2023683758 doi "https://doi.org/10.1016/s0969-2126(98)00132-4" @default.
• W2023683758 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9782055" @default.
• W2023683758 hasPublicationYear "1998" @default.
• W2023683758 type Work @default.
• W2023683758 sameAs 2023683758 @default.
• W2023683758 citedByCount "41" @default.
• W2023683758 countsByYear W20236837582012 @default.
• W2023683758 countsByYear W20236837582015 @default.
• W2023683758 countsByYear W20236837582016 @default.
• W2023683758 countsByYear W20236837582017 @default.
• W2023683758 countsByYear W20236837582018 @default.
• W2023683758 countsByYear W20236837582019 @default.
• W2023683758 countsByYear W20236837582022 @default.
• W2023683758 crossrefType "journal-article" @default.
• W2023683758 hasAuthorship W2023683758A5007964042 @default.
• W2023683758 hasAuthorship W2023683758A5050678734 @default.
• W2023683758 hasAuthorship W2023683758A5061217668 @default.
• W2023683758 hasBestOaLocation W20236837581 @default.
• W2023683758 hasConcept C104317684 @default.
• W2023683758 hasConcept C139489369 @default.
• W2023683758 hasConcept C150194340 @default.
• W2023683758 hasConcept C167625842 @default.
• W2023683758 hasConcept C181199279 @default.
• W2023683758 hasConcept C185592680 @default.
• W2023683758 hasConcept C192772702 @default.
• W2023683758 hasConcept C195286587 @default.
• W2023683758 hasConcept C2779485144 @default.
• W2023683758 hasConcept C2780120296 @default.
• W2023683758 hasConcept C41183919 @default.
• W2023683758 hasConcept C45484198 @default.
• W2023683758 hasConcept C47701112 @default.
• W2023683758 hasConcept C54355233 @default.
• W2023683758 hasConcept C547475151 @default.
• W2023683758 hasConcept C55493867 @default.
• W2023683758 hasConcept C71240020 @default.
• W2023683758 hasConcept C86803240 @default.
• W2023683758 hasConceptScore W2023683758C104317684 @default.
• W2023683758 hasConceptScore W2023683758C139489369 @default.
• W2023683758 hasConceptScore W2023683758C150194340 @default.
• W2023683758 hasConceptScore W2023683758C167625842 @default.
• W2023683758 hasConceptScore W2023683758C181199279 @default.
• W2023683758 hasConceptScore W2023683758C185592680 @default.
• W2023683758 hasConceptScore W2023683758C192772702 @default.
• W2023683758 hasConceptScore W2023683758C195286587 @default.
• W2023683758 hasConceptScore W2023683758C2779485144 @default.
• W2023683758 hasConceptScore W2023683758C2780120296 @default.
• W2023683758 hasConceptScore W2023683758C41183919 @default.
• W2023683758 hasConceptScore W2023683758C45484198 @default.
• W2023683758 hasConceptScore W2023683758C47701112 @default.
• W2023683758 hasConceptScore W2023683758C54355233 @default.
• W2023683758 hasConceptScore W2023683758C547475151 @default.
• W2023683758 hasConceptScore W2023683758C55493867 @default.
• W2023683758 hasConceptScore W2023683758C71240020 @default.
• W2023683758 hasConceptScore W2023683758C86803240 @default.

• next