FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023691907> ?p ?o ?g. }

• W2023691907 endingPage "873" @default.
• W2023691907 startingPage "867" @default.
• W2023691907 abstract "The crystallographic structure of the p53 core domain showed that most of the p53 mutations found in human tumors are located in conserved regions of the p53 DNA-binding domain. The aim of our study was to investigate the effect on DNA-binding and transactivation of three p53 mutations frequently found in hepatocellular carcinomas (HCC). Two of these mutations are located near the DNA-binding surface and are induced by aflatoxin B1 (249ser) and oxiradicals (249met). In contrast, mutation 220cys is not associated with a specific carcinogen in HCCs and is located outside the DNA binding structures of p53. Cotransfection experiments in two HCC cell lines, with mutated or deleted P53 genes, showed that all three mutations did not enhance reporter gene activity (RGC-CAT), in contrast to wt p53. However, in hepatoma cell lines all three mutations did suppress the p53 wildtype (wt) transactivation in a dose-dependent fashion. DNA-binding was monitored by gel shift assays using the consensus-, Waf-, and RGC-p53 binding sites. All three p53 mutations did decrease DNA-binding versus all binding sites included. Interestingly although all mutations showed the same DNA-binding and transactivation properties, differences in the ectopic expression in different hepatoma cells were observed. Therefore our results indicate that p53 mutations in HCC found in the DNA-binding domain and outside the conserved DNA-binding structures modulate target gene expression by decreasing sequence specific DNA-binding in a dominant negative fashion. The cellular environment may contribute to an additional selection advantage of some mutations." @default.
• W2023691907 created "2016-06-24" @default.
• W2023691907 creator A5002183557 @default.
• W2023691907 creator A5006242340 @default.
• W2023691907 creator A5047515302 @default.
• W2023691907 creator A5024847937 @default.
• W2023691907 date "1997-04-01" @default.
• W2023691907 modified "2023-10-06" @default.
• W2023691907 title "Target gene modulation in hepatocellular carcinomas by decreased DNA-binding of p53 mutations" @default.
• W2023691907 cites W1483541096 @default.
• W2023691907 cites W1520465138 @default.
• W2023691907 cites W1552426876 @default.
• W2023691907 cites W1554072136 @default.
• W2023691907 cites W1555236883 @default.
• W2023691907 cites W156497418 @default.
• W2023691907 cites W1575805367 @default.
• W2023691907 cites W1600706591 @default.
• W2023691907 cites W1862543179 @default.
• W2023691907 cites W1973368924 @default.
• W2023691907 cites W1978400153 @default.
• W2023691907 cites W1989936068 @default.
• W2023691907 cites W1991961027 @default.
• W2023691907 cites W1993475388 @default.
• W2023691907 cites W1995856480 @default.
• W2023691907 cites W2005546492 @default.
• W2023691907 cites W2013136213 @default.
• W2023691907 cites W2028929505 @default.
• W2023691907 cites W2034200840 @default.
• W2023691907 cites W2034349902 @default.
• W2023691907 cites W2035794058 @default.
• W2023691907 cites W2042316019 @default.
• W2023691907 cites W2050793927 @default.
• W2023691907 cites W2057708151 @default.
• W2023691907 cites W206297210 @default.
• W2023691907 cites W2068691401 @default.
• W2023691907 cites W2068889468 @default.
• W2023691907 cites W2095243258 @default.
• W2023691907 cites W2096276671 @default.
• W2023691907 cites W2103057278 @default.
• W2023691907 cites W2136786699 @default.
• W2023691907 cites W2164298548 @default.
• W2023691907 cites W2223661640 @default.
• W2023691907 cites W2272408415 @default.
• W2023691907 cites W2278542555 @default.
• W2023691907 cites W2300675664 @default.
• W2023691907 cites W2340162342 @default.
• W2023691907 cites W2402486682 @default.
• W2023691907 cites W2405173083 @default.
• W2023691907 cites W2407949018 @default.
• W2023691907 cites W2443584055 @default.
• W2023691907 cites W2470617693 @default.
• W2023691907 cites W2472494377 @default.
• W2023691907 cites W1753185091 @default.
• W2023691907 doi "https://doi.org/10.1002/hep.510250414" @default.
• W2023691907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9096590" @default.
• W2023691907 hasPublicationYear "1997" @default.
• W2023691907 type Work @default.
• W2023691907 sameAs 2023691907 @default.
• W2023691907 citedByCount "22" @default.
• W2023691907 countsByYear W20236919072012 @default.
• W2023691907 countsByYear W20236919072014 @default.
• W2023691907 countsByYear W20236919072015 @default.
• W2023691907 countsByYear W20236919072017 @default.
• W2023691907 countsByYear W20236919072022 @default.
• W2023691907 countsByYear W20236919072023 @default.
• W2023691907 crossrefType "journal-article" @default.
• W2023691907 hasAuthorship W2023691907A5002183557 @default.
• W2023691907 hasAuthorship W2023691907A5006242340 @default.
• W2023691907 hasAuthorship W2023691907A5024847937 @default.
• W2023691907 hasAuthorship W2023691907A5047515302 @default.
• W2023691907 hasBestOaLocation W20236919071 @default.
• W2023691907 hasConcept C101762097 @default.
• W2023691907 hasConcept C104317684 @default.
• W2023691907 hasConcept C107824862 @default.
• W2023691907 hasConcept C1292079 @default.
• W2023691907 hasConcept C150194340 @default.
• W2023691907 hasConcept C153911025 @default.
• W2023691907 hasConcept C25166345 @default.
• W2023691907 hasConcept C2779314089 @default.
• W2023691907 hasConcept C33987129 @default.
• W2023691907 hasConcept C3662595 @default.
• W2023691907 hasConcept C501734568 @default.
• W2023691907 hasConcept C502942594 @default.
• W2023691907 hasConcept C5179208 @default.
• W2023691907 hasConcept C54355233 @default.
• W2023691907 hasConcept C552990157 @default.
• W2023691907 hasConcept C86339819 @default.
• W2023691907 hasConcept C86803240 @default.
• W2023691907 hasConcept C94966510 @default.
• W2023691907 hasConcept C97037327 @default.
• W2023691907 hasConceptScore W2023691907C101762097 @default.
• W2023691907 hasConceptScore W2023691907C104317684 @default.
• W2023691907 hasConceptScore W2023691907C107824862 @default.
• W2023691907 hasConceptScore W2023691907C1292079 @default.
• W2023691907 hasConceptScore W2023691907C150194340 @default.
• W2023691907 hasConceptScore W2023691907C153911025 @default.
• W2023691907 hasConceptScore W2023691907C25166345 @default.
• W2023691907 hasConceptScore W2023691907C2779314089 @default.

• next