Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023695875> ?p ?o ?g. }
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- W2023695875 abstract "During early mammalian development, blastocyst morphogenesis is achieved by epithelial differentiation of trophectoderm (TE) and its segregation from the inner cell mass (ICM). Two major interrelated features of TE differentiation required for blastocoel formation include intercellular junction biogenesis and a directed ion transport system, mediated by Na+/K+ ATPase. We have examined the relative contribution of intercellular signalling mediated by protein kinase C (PKC) and gap junctional communication in TE differentiation and blastocyst cavitation. The distribution pattern of four (δ, θ, ι/λ, ζ) PKC isoforms and PKCμ/PKD1 showed partial colocalisation with the tight junction marker ZO-1α+ in TE and all four PKCs (δ, θ, ι/λ, ζ) showed distinct TE/ICM staining patterns (predominantly at the cell membrane within the TE and cytoplasmic within the ICM), indicating their potential contribution to TE differentiation and blastocyst morphogenesis. Specific inhibition of PKCδ and ζ activity significantly delayed blastocyst formation. Although modulation of these PKC isoforms failed to influence the already established programme of epithelial junctional differentiation within the TE, Na+/K+ ATPase α1 subunit was internalised from membrane to cytoplasm. Inhibition of gap junctional communication, in contrast, had no influence on any of these processes. Our results demonstrate for the first time that distinct PKC isotypes contribute to the regulation of cavitation in preimplantation embryos via target proteins including Na+/K+ ATPase." @default.
- W2023695875 created "2016-06-24" @default.
- W2023695875 creator A5007815424 @default.
- W2023695875 creator A5018183258 @default.
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- W2023695875 creator A5032042476 @default.
- W2023695875 creator A5044904465 @default.
- W2023695875 creator A5071034818 @default.
- W2023695875 date "2004-10-01" @default.
- W2023695875 modified "2023-10-14" @default.
- W2023695875 title "Specific PKC isoforms regulate blastocoel formation during mouse preimplantation development" @default.
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