FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023732026> ?p ?o ?g. }

• W2023732026 endingPage "012034" @default.
• W2023732026 startingPage "012034" @default.
• W2023732026 abstract "Nanotechnology is a broad, revolutionary field with promising advantages for new medicine. In this context the rapid development and improvement of so called nanocarriers is of high pharmaceutical interest and some devices are already on the market. In our project we aim to develop well characterized nanoscaled drug delivery systems for an inhalative application. To this end, we focus on the most adverse side-effects within the lung, the cytotoxic and the proinflammatory responses to these nanoparticles (NPs). Before performing any animal experiments, we start with an in vitro screening for analyzing the cytotoxic and proinflammatory effects of the investigated particles on two murine lung target cell lines, the alveolar epithelial like typ II cell line (LA4) and the alveolar macrophage cell line (MH-S). Three different endpoints were estimated, (i) cellular metabolic activity, determined by the WST-1 assay, (ii) membrane integrity, by detection of LDH release and hemolytic activity, and (iii) secretion of inflammatory mediators. To analyze the relative particle toxicity we choose two reference particles as benchmarks, (i) fine a-quartz, and (ii) ultrafine ZnO particles. The investigation of dose-response and kinetics of proinflammatory and toxic effects caused to the named cell lines provide an insight to a close evaluation of our cell based screening strategy. oc-quartz is well known for its inflammatory and toxic potential caused by inhalation, and nanosized ZnO particles - used in a broad field of nanotechnology like electronics, but also cosmetics and pharmaceuticals - is to a high degree cytotoxic and proinflammatory in vitro. Preliminary experiments indicated not only particle and cell specific inflammatory responses, but also different susceptibilities of the cell types being exposed to our benchmark particles regarding their size and surface activities. Exposure to the μm-sized a-quartz particles affected the viability of epithelia cells less than that of macrophages, pointing to the impact of particle uptake by phagocytosis. In contrast, the nanosized ZnO particles caused much stronger decrease in cell viability and higher levels of LDH in the macrophage cell line compared to epithelial cells, even though the hemolytic activity was much higher for the a-quartz particles than for the nanosized ZnO. For the proinflammatory effects, we observed a clear dose-dependent release of acute phase cytokines (TNF-α, IL-6, G-CSF> CXCL10>CCL2) for both alveolar cell lines after Min-U-Sil exposure. After ZnO treatment the cytokine responses were negligible compare to control cells. In conclusion, our data attach value to the use of different cell types to detect different pathways of toxicity generated by different particle properties. Therefore, we will establish both lung target cell lines for an in vitro screening to analyze proinflammatory and cytotoxicity effects of nanocarriers. The implementation of the two reference particles facilitate the validated classification of the cytotoxic responses caused by the NPs investigated." @default.
• W2023732026 created "2016-06-24" @default.
• W2023732026 creator A5000222507 @default.
• W2023732026 creator A5017318583 @default.
• W2023732026 creator A5056150994 @default.
• W2023732026 creator A5090579172 @default.
• W2023732026 date "2009-02-01" @default.
• W2023732026 modified "2023-09-26" @default.
• W2023732026 title "Screening strategy to avoid toxicological hazards of inhaled nanoparticles for drug delivery: The use of a-quartz and nano zinc oxide particles as benchmark" @default.
• W2023732026 cites W1966453354 @default.
• W2023732026 cites W1966476051 @default.
• W2023732026 cites W1967075348 @default.
• W2023732026 cites W1982454784 @default.
• W2023732026 cites W2007086477 @default.
• W2023732026 cites W2011753513 @default.
• W2023732026 cites W2024481637 @default.
• W2023732026 cites W2027404821 @default.
• W2023732026 cites W2049382198 @default.
• W2023732026 cites W2078377919 @default.
• W2023732026 cites W2086069830 @default.
• W2023732026 cites W2100600921 @default.
• W2023732026 cites W2103449823 @default.
• W2023732026 cites W2114918609 @default.
• W2023732026 cites W2140720810 @default.
• W2023732026 cites W2163577514 @default.
• W2023732026 doi "https://doi.org/10.1088/1742-6596/151/1/012034" @default.
• W2023732026 hasPublicationYear "2009" @default.
• W2023732026 type Work @default.
• W2023732026 sameAs 2023732026 @default.
• W2023732026 citedByCount "13" @default.
• W2023732026 countsByYear W20237320262012 @default.
• W2023732026 countsByYear W20237320262013 @default.
• W2023732026 countsByYear W20237320262014 @default.
• W2023732026 countsByYear W20237320262015 @default.
• W2023732026 countsByYear W20237320262016 @default.
• W2023732026 countsByYear W20237320262019 @default.
• W2023732026 countsByYear W20237320262020 @default.
• W2023732026 countsByYear W20237320262021 @default.
• W2023732026 countsByYear W20237320262023 @default.
• W2023732026 crossrefType "journal-article" @default.
• W2023732026 hasAuthorship W2023732026A5000222507 @default.
• W2023732026 hasAuthorship W2023732026A5017318583 @default.
• W2023732026 hasAuthorship W2023732026A5056150994 @default.
• W2023732026 hasAuthorship W2023732026A5090579172 @default.
• W2023732026 hasBestOaLocation W20237320261 @default.
• W2023732026 hasConcept C127445965 @default.
• W2023732026 hasConcept C151730666 @default.
• W2023732026 hasConcept C155672457 @default.
• W2023732026 hasConcept C164027704 @default.
• W2023732026 hasConcept C171250308 @default.
• W2023732026 hasConcept C178790620 @default.
• W2023732026 hasConcept C18150654 @default.
• W2023732026 hasConcept C185592680 @default.
• W2023732026 hasConcept C192562407 @default.
• W2023732026 hasConcept C203014093 @default.
• W2023732026 hasConcept C2776914184 @default.
• W2023732026 hasConcept C2779343474 @default.
• W2023732026 hasConcept C2779820397 @default.
• W2023732026 hasConcept C29730261 @default.
• W2023732026 hasConcept C71924100 @default.
• W2023732026 hasConcept C86803240 @default.
• W2023732026 hasConceptScore W2023732026C127445965 @default.
• W2023732026 hasConceptScore W2023732026C151730666 @default.
• W2023732026 hasConceptScore W2023732026C155672457 @default.
• W2023732026 hasConceptScore W2023732026C164027704 @default.
• W2023732026 hasConceptScore W2023732026C171250308 @default.
• W2023732026 hasConceptScore W2023732026C178790620 @default.
• W2023732026 hasConceptScore W2023732026C18150654 @default.
• W2023732026 hasConceptScore W2023732026C185592680 @default.
• W2023732026 hasConceptScore W2023732026C192562407 @default.
• W2023732026 hasConceptScore W2023732026C203014093 @default.
• W2023732026 hasConceptScore W2023732026C2776914184 @default.
• W2023732026 hasConceptScore W2023732026C2779343474 @default.
• W2023732026 hasConceptScore W2023732026C2779820397 @default.
• W2023732026 hasConceptScore W2023732026C29730261 @default.
• W2023732026 hasConceptScore W2023732026C71924100 @default.
• W2023732026 hasConceptScore W2023732026C86803240 @default.
• W2023732026 hasLocation W20237320261 @default.
• W2023732026 hasOpenAccess W2023732026 @default.
• W2023732026 hasPrimaryLocation W20237320261 @default.
• W2023732026 hasRelatedWork W1595862141 @default.
• W2023732026 hasRelatedWork W2287489801 @default.
• W2023732026 hasRelatedWork W2547388630 @default.
• W2023732026 hasRelatedWork W2791060554 @default.
• W2023732026 hasRelatedWork W2902965611 @default.
• W2023732026 hasRelatedWork W2980574643 @default.
• W2023732026 hasRelatedWork W3006014150 @default.
• W2023732026 hasRelatedWork W3107885797 @default.
• W2023732026 hasRelatedWork W3141710119 @default.
• W2023732026 hasRelatedWork W4311495042 @default.
• W2023732026 hasVolume "151" @default.
• W2023732026 isParatext "false" @default.
• W2023732026 isRetracted "false" @default.
• W2023732026 magId "2023732026" @default.
• W2023732026 workType "article" @default.