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- W2023741839 abstract "CD40 ligand (CD40L) is a member of the tumor necrosis factor superfamily and is produced in a variety of cells, including platelets. The soluble form (sCD40L) is a mediator of both inflammatory and hemostasis processes and has been implicated in the pathogenesis of atherosclerosis. Clinical studies have revealed increased sCD40L in patients with unstable angina (1) and identified an association between increased sCD40L and future risk for death or nonfatal myocardial infarction (2)(3). While prospectively measuring sCD40L in a cohort of persons at risk for cardiovascular complications, we identified both preanalytical and analytical sources of error. This report documents the effects of specimen type (serum and plasma), processing (time and temperature), and commercial reagent selection on sCD40L ELISA results. These findings raise concerns about the accuracy of sCD40L results reported in recent clinical studies.After obtaining informed consent, we enrolled 147 patients older than 60 years referred for diagnostic cardiac catheterization in an Institutional Review Board-approved study to evaluate the value of clinical, echocardiographic, and biomarker variables for prediction of future cardiovascular complications. When combined with clinical predictors, B-type natriuretic peptide and C-reactive protein, but not sCD40L, were independent predictors of death or cardiovascular hospitalization at 6 months (data not shown). The unexpectedly poor correlation between undiluted plasma sCD40L results and clinical outcomes in this study motivated us to perform the following investigations.Venous blood from the 147 study participants [mean (SD) age, 70.8 (6.9) years] was collected into plastic tubes containing tripotassium EDTA (BD Vacutainer; Becton Dickinson) before catheterization and placed on ice for 1–4 h before processing. Blood from 10 control individuals [mean (SD) age, 38.7 (8.4) years] was collected into both EDTA and plain glass tubes (Becton Dickinson) and maintained at room temperature for 30 min before processing. Study and control samples were centrifuged twice: …" @default.
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- W2023741839 date "2005-06-01" @default.
- W2023741839 modified "2023-10-18" @default.
- W2023741839 title "Soluble CD40 Ligand Measurement Inaccuracies Attributable to Specimen Type, Processing Time, and ELISA Method" @default.
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- W2023741839 doi "https://doi.org/10.1373/clinchem.2005.048199" @default.
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