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- W2023754290 abstract "The specificity of a dialyzable component, isolated from rabbit reticulocyte initiation factors, in stimulating protein synthesis was examined. It appeard that his factor (hereafter designated as iRNA) was able to restore the activity of initiation factor preparations that were inactivated by dialysis. The iRNA from reticulocytes stimulated polypeptide synthesis directed by the homologous globin mRNA as well as heterologous lens crystallin mRNAs. No selectivity in its stimulating action with regard to the type of mRNA studied could be observed. The source of ribosomes or supernatant enzymes did not influence the effect of iRNA. However, in an ascites lysate that was dependent on the addition of initiation factors, iRNA increased polypeptide formation only marginally, suggesting that in this lysate a similar factor was already present in an active form. It is concluded that iRNA may regulate protein synthesis, but without exhibiting specificity towards mRNAs, at least those tested so far." @default.
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- W2023754290 date "1975-02-01" @default.
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- W2023754290 title "Non-specific stimulation of cell-free protein synthesis by a dialyzable factor isolated from reticulocyte initiation factors (iRNA)." @default.
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- W2023754290 doi "https://doi.org/10.1073/pnas.72.2.714" @default.
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