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- W2023755320 abstract "Objective: To evaluate the role of CD19+CD5+ B-1a B cells in controlling Th1 and Th17 T cell differentiation during pregnancy using a mouse model of pregnancy disturbance. Methods: CD19+CD5+ cells were magnetically isolated from the peritoneal cavity (PerC) of non-pregnant, BALB/cmated (normal pregnant combination [NP]) or DBA/2J mated (abortion-prone combination [AP]) CBA/J females on day 14 of pregnancy. Isolated cells were inactivated with mitomycin-c and co-cultured (1:2) for 5 days with magnetically isolated allogeneic CD4+CD25− T cells. Some wells were treated with a Th17 differentiation cocktail (anti-IFN , anti-IL4, TGF , IL6, and IL23). Th1 and Th17 cytokines in supernatants were measured by CBA array system. Expression levels of B-1a B cell activation markers (MHCII, CD80, CD86, PDL1, PDL2 and FASL) were assessed in B-1a B cells from non-pregnant, NP and AP mice. Results: B-1a B cells from non-pregnantmice induced a modest productionof IL17byT cells thatwasboostedwhen T cells were co-cultured with B-1a B cells from AP mice. Notably, B-1a B cells from NP mice strongly inhibited IL17 production by T cells, even in the presence of a Th17 induction cocktail. Similarly, Th1 cytokines (TNF, IFN, IL2 and MCP-1) were higher produced by T cells when co-cultured with B-1a B cells from AP mice compared with those cultured with B-1a B cells from NP mice. B-1a B cells from NP mice expressed significantly lower levels of CD86 thanB-1a B cells from AP or non-pregnant control mice. Conclusion:We demonstrated in this work that B-1a B cells play a central role in regulating T cell differentiation associated with pregnancy disturbances. This unexplored role of B-1a B cells opens up new alternatives to design strategies to control undesired immuneactivationaffecting pregnancy outcome." @default.
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- W2023755320 date "2014-03-01" @default.
- W2023755320 modified "2023-09-26" @default.
- W2023755320 title "Effect of Intralipid on some NK and T-cells activation markers" @default.
- W2023755320 doi "https://doi.org/10.1016/j.jri.2013.12.040" @default.
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