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- W2023761946 abstract "When hydroxo-vitamin B12, which predominates in foods, is treated by microwave heating, two degradation products with Rf of 0.16 and 0.27 are found and characterized (Watanabe et al. J. Agric. Food Chem. 1998, 46, 206−210). A novel hydroxo-vitamin B12 degradation product with a Rf of 0.12 was also found by the use of silica gel 60 column chromatography and was the major compound among the degradation products. The degradation product with a Rf of 0.12 was purified to homogeneity and partially characterized. The purified degradation product had about 13% and 23% biological activity of authentic vitamin B12 in hog intrinsic factor (a mammalian vitamin B12-binding protein) and Lactobacillus leichmannii ATCC 7830, respectively. Administration of the purified degradation product (0.5 μg/week) to rats indicates that the compound does not have significant activity in mammals. Keywords: Vitamin B12; degradation; microwave heating; intrinsic factor; Lactobacillus leichmannii; mammalian cells; rat" @default.
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- W2023761946 date "1998-11-20" @default.
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- W2023761946 title "Biological Activity of Hydroxo-vitamin B12 Degradation Product Formed during Microwave Heating" @default.
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- W2023761946 doi "https://doi.org/10.1021/jf980727v" @default.
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