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- W2023762375 abstract "Intracellular free calcium ([Ca2+]i) was measured in individual pancreatic β-cells from mice using dual emission microfluorometry and the indicator Indo-1 applied by a patch clamp pipette. GTP-γ-S (100 μM) injected together with 0.3 or 3 mM ATP evoked repetitive [Ca2+]i transients with a frequency of about 1 per min in β-cells kept at a membrane potential of −70 mV. The oscillatory pattern was unaffected by the Ca2+ channel blocker verapamil (50 μM). When omitting GTP-γ-S from the pipette medium it became evident that 3 mM ATP alone can induce oscillations. The results provide additional evidence for an important role of ATP in the ionic control of insulin release, indicating that such regulation may also involve activation of G-proteins." @default.
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- W2023762375 title "Intracellular ATP mimics GTP-γ-S in generating Ca2+ oscillations in pancreatic β-cells" @default.
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- W2023762375 doi "https://doi.org/10.1016/0006-291x(91)91856-8" @default.
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