FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023768033> ?p ?o ?g. }

• W2023768033 endingPage "46" @default.
• W2023768033 startingPage "36" @default.
• W2023768033 abstract "Background Transcription factors play essential roles in both normal and malignant hematopoiesis. This is the case for the growth factor independent 1b (GFI1B) transcription factor, which is required for erythroid and megakaryocytic differentiation and over-expressed in leukemic patients and cell lines.Design and Methods To investigate GFI1B regulation, we searched for multispecies conserved non-coding elements between GFI1B and neighboring genes. We used a formaldehyde-assisted isolation of regulatory elements (FAIRE) assay and DNase1 hypersensitivity to assess the chromatin conformation of these sites. Next, we analyzed transcription factor binding and histone modifications at the GFI1B locus including the conserved non-coding elements by a chromatin immunoprecipitation assay. Finally, we studied the interaction of the GFI1B promoter and the conserved non-coding elements with the chromatin conformation capture technique and used immunofluorescence to evaluate GFI1B levels in individual cells.Results We localized several conserved non-coding elements containing multiple erythroid specific transcription factor binding sites at the GFI1B locus. In GFI1B-expressing cells a subset of these conserved non-coding elements and the promoter adopt a close spatial conformation, localize with open chromatin sites, harbor chromatin modifications associated with gene activation and bind multiple transcription factors and co-repressors.Conclusions Our findings indicate that GFI1B regulatory elements behave as activators and repressors. Different protein levels within a cell population suggest that cells must activate and repress GFI1B continuously to control its final level. These data are consistent with a model of GFI1B regulation in which GFI1B binds to its own promoter and to the conserved non-coding elements as its levels rise. This would attract repressor complexes that progressively down-regulate the gene. GFI1B expression would decrease until a stage at which the activating complexes predominate and expression increases." @default.
• W2023768033 created "2016-06-24" @default.
• W2023768033 creator A5034664822 @default.
• W2023768033 creator A5038457206 @default.
• W2023768033 creator A5075260593 @default.
• W2023768033 creator A5087068324 @default.
• W2023768033 date "2009-09-22" @default.
• W2023768033 modified "2023-09-25" @default.
• W2023768033 title "GFI1B controls its own expression binding to multiple sites" @default.
• W2023768033 cites W134056315 @default.
• W2023768033 cites W1496551672 @default.
• W2023768033 cites W1561219581 @default.
• W2023768033 cites W1967255711 @default.
• W2023768033 cites W1967894253 @default.
• W2023768033 cites W1970081233 @default.
• W2023768033 cites W1973049010 @default.
• W2023768033 cites W1974200066 @default.
• W2023768033 cites W1977842421 @default.
• W2023768033 cites W1981101983 @default.
• W2023768033 cites W1984327907 @default.
• W2023768033 cites W1998536026 @default.
• W2023768033 cites W2002698073 @default.
• W2023768033 cites W2004232360 @default.
• W2023768033 cites W2012170788 @default.
• W2023768033 cites W2023076106 @default.
• W2023768033 cites W2023303970 @default.
• W2023768033 cites W2025589527 @default.
• W2023768033 cites W2026367093 @default.
• W2023768033 cites W2028553942 @default.
• W2023768033 cites W2030975965 @default.
• W2023768033 cites W2042323308 @default.
• W2023768033 cites W2056264836 @default.
• W2023768033 cites W2056846219 @default.
• W2023768033 cites W2058223580 @default.
• W2023768033 cites W2070794112 @default.
• W2023768033 cites W2089019735 @default.
• W2023768033 cites W2089894746 @default.
• W2023768033 cites W2094956978 @default.
• W2023768033 cites W2097184672 @default.
• W2023768033 cites W2100726754 @default.
• W2023768033 cites W2107277218 @default.
• W2023768033 cites W2109719547 @default.
• W2023768033 cites W2111885793 @default.
• W2023768033 cites W2113116005 @default.
• W2023768033 cites W2115686505 @default.
• W2023768033 cites W2116608195 @default.
• W2023768033 cites W2119833870 @default.
• W2023768033 cites W2136317405 @default.
• W2023768033 cites W2136352982 @default.
• W2023768033 cites W2138891173 @default.
• W2023768033 cites W2141478246 @default.
• W2023768033 cites W2143927198 @default.
• W2023768033 cites W2144473036 @default.
• W2023768033 cites W2146652917 @default.
• W2023768033 cites W2156083214 @default.
• W2023768033 cites W2159680972 @default.
• W2023768033 cites W2162198627 @default.
• W2023768033 cites W2162912283 @default.
• W2023768033 cites W2166647372 @default.
• W2023768033 cites W2170064419 @default.
• W2023768033 doi "https://doi.org/10.3324/haematol.2009.012351" @default.
• W2023768033 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2805738" @default.
• W2023768033 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19773260" @default.
• W2023768033 hasPublicationYear "2009" @default.
• W2023768033 type Work @default.
• W2023768033 sameAs 2023768033 @default.
• W2023768033 citedByCount "25" @default.
• W2023768033 countsByYear W20237680332012 @default.
• W2023768033 countsByYear W20237680332013 @default.
• W2023768033 countsByYear W20237680332014 @default.
• W2023768033 countsByYear W20237680332015 @default.
• W2023768033 countsByYear W20237680332016 @default.
• W2023768033 countsByYear W20237680332017 @default.
• W2023768033 countsByYear W20237680332018 @default.
• W2023768033 countsByYear W20237680332019 @default.
• W2023768033 countsByYear W20237680332020 @default.
• W2023768033 countsByYear W20237680332021 @default.
• W2023768033 countsByYear W20237680332023 @default.
• W2023768033 crossrefType "journal-article" @default.
• W2023768033 hasAuthorship W2023768033A5034664822 @default.
• W2023768033 hasAuthorship W2023768033A5038457206 @default.
• W2023768033 hasAuthorship W2023768033A5075260593 @default.
• W2023768033 hasAuthorship W2023768033A5087068324 @default.
• W2023768033 hasBestOaLocation W20237680331 @default.
• W2023768033 hasConcept C101762097 @default.
• W2023768033 hasConcept C104317684 @default.
• W2023768033 hasConcept C134320426 @default.
• W2023768033 hasConcept C138885662 @default.
• W2023768033 hasConcept C150194340 @default.
• W2023768033 hasConcept C153911025 @default.
• W2023768033 hasConcept C158448853 @default.
• W2023768033 hasConcept C167625842 @default.
• W2023768033 hasConcept C179926584 @default.
• W2023768033 hasConcept C199216141 @default.
• W2023768033 hasConcept C41895202 @default.
• W2023768033 hasConcept C54355233 @default.
• W2023768033 hasConcept C83640560 @default.
• W2023768033 hasConcept C86339819 @default.

• next