Matches in SemOpenAlex for { <https://semopenalex.org/work/W2023771275> ?p ?o ?g. }
- W2023771275 endingPage "1260" @default.
- W2023771275 startingPage "1250" @default.
- W2023771275 abstract "The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared proteomes of tissue cultures from leaf explants of these two lines. Both 2HA and Jemalong explants were grown on media containing the auxin 1-naphthaleneacetic acid and the cytokinin 6-benzylaminopurine. Proteins were extracted from the cultures at different time points (2, 5, and 8 weeks), separated by two-dimensional gel electrophoresis, and detected by silver staining. More than 2,000 proteins could be reproducibly resolved and detected on each gel. Statistical analysis showed that 54 protein spots were significantly (P < 0.05) changed in expression (accumulation) during the 8 weeks of culture, and most of these spots were extracted from colloidal Coomassie-stained two-dimensional gel electrophoresis gels and were subjected to matrix-assisted laser desorption ionization time-of-flight mass spectrometry or liquid chromatography-tandem mass spectrometry analysis. Using a publicly available expressed sequence tag database and the Mascot search engine, we were able to identify 16 differentially expressed proteins. More than 60% of the differentially expressed protein spots had very different patterns of gene expression between 2HA and Jemalong during the 8 weeks of culture." @default.
- W2023771275 created "2016-06-24" @default.
- W2023771275 creator A5001919234 @default.
- W2023771275 creator A5001992219 @default.
- W2023771275 creator A5003185536 @default.
- W2023771275 creator A5006615829 @default.
- W2023771275 creator A5045078388 @default.
- W2023771275 creator A5065716508 @default.
- W2023771275 date "2005-04-01" @default.
- W2023771275 modified "2023-09-28" @default.
- W2023771275 title "Proteomic Analysis of Somatic Embryogenesis in <i>Medicago truncatula</i>. Explant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments" @default.
- W2023771275 cites W103579110 @default.
- W2023771275 cites W1527834429 @default.
- W2023771275 cites W1595716614 @default.
- W2023771275 cites W1936182333 @default.
- W2023771275 cites W1963487450 @default.
- W2023771275 cites W1969111385 @default.
- W2023771275 cites W1976733777 @default.
- W2023771275 cites W1982665799 @default.
- W2023771275 cites W1989762240 @default.
- W2023771275 cites W1995224698 @default.
- W2023771275 cites W1995310701 @default.
- W2023771275 cites W1997873541 @default.
- W2023771275 cites W1999044492 @default.
- W2023771275 cites W1999281364 @default.
- W2023771275 cites W1999634878 @default.
- W2023771275 cites W2002724826 @default.
- W2023771275 cites W2005057009 @default.
- W2023771275 cites W2006556103 @default.
- W2023771275 cites W2007901709 @default.
- W2023771275 cites W2008081009 @default.
- W2023771275 cites W2011404532 @default.
- W2023771275 cites W2020293054 @default.
- W2023771275 cites W2029504050 @default.
- W2023771275 cites W2032661681 @default.
- W2023771275 cites W2034119887 @default.
- W2023771275 cites W2035514342 @default.
- W2023771275 cites W2035932370 @default.
- W2023771275 cites W2036775866 @default.
- W2023771275 cites W2041706717 @default.
- W2023771275 cites W2042117874 @default.
- W2023771275 cites W2045855357 @default.
- W2023771275 cites W2048223003 @default.
- W2023771275 cites W2049658044 @default.
- W2023771275 cites W2052027914 @default.
- W2023771275 cites W2056702772 @default.
- W2023771275 cites W2058196275 @default.
- W2023771275 cites W2059687544 @default.
- W2023771275 cites W2072213305 @default.
- W2023771275 cites W2073262239 @default.
- W2023771275 cites W2074277812 @default.
- W2023771275 cites W2075324691 @default.
- W2023771275 cites W2076950551 @default.
- W2023771275 cites W2087975443 @default.
- W2023771275 cites W2088632260 @default.
- W2023771275 cites W2089080937 @default.
- W2023771275 cites W2094398627 @default.
- W2023771275 cites W2097353843 @default.
- W2023771275 cites W2097752593 @default.
- W2023771275 cites W2098149915 @default.
- W2023771275 cites W2102315416 @default.
- W2023771275 cites W2104412732 @default.
- W2023771275 cites W2106547178 @default.
- W2023771275 cites W2106756197 @default.
- W2023771275 cites W2111491687 @default.
- W2023771275 cites W2113057324 @default.
- W2023771275 cites W2116814217 @default.
- W2023771275 cites W2120373397 @default.
- W2023771275 cites W2128251929 @default.
- W2023771275 cites W2130132005 @default.
- W2023771275 cites W2130348401 @default.
- W2023771275 cites W2130578985 @default.
- W2023771275 cites W2137042863 @default.
- W2023771275 cites W2143929056 @default.
- W2023771275 cites W2148192995 @default.
- W2023771275 cites W2148750035 @default.
- W2023771275 cites W2151090719 @default.
- W2023771275 cites W2154242666 @default.
- W2023771275 cites W2156819162 @default.
- W2023771275 cites W2161012844 @default.
- W2023771275 cites W2161657088 @default.
- W2023771275 cites W2162526240 @default.
- W2023771275 cites W2164510037 @default.
- W2023771275 cites W2164901649 @default.
- W2023771275 cites W2334344722 @default.
- W2023771275 cites W277570634 @default.
- W2023771275 cites W4211202133 @default.
- W2023771275 doi "https://doi.org/10.1104/pp.104.055277" @default.
- W2023771275 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1088318" @default.
- W2023771275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15749990" @default.
- W2023771275 hasPublicationYear "2005" @default.
- W2023771275 type Work @default.
- W2023771275 sameAs 2023771275 @default.
- W2023771275 citedByCount "123" @default.
- W2023771275 countsByYear W20237712752012 @default.
- W2023771275 countsByYear W20237712752013 @default.
- W2023771275 countsByYear W20237712752014 @default.
- W2023771275 countsByYear W20237712752015 @default.