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- W2023772106 abstract "Mg2+ gave dose-dependent activation of 5-lipoxygenase (5LO) in vitro. As for Ca2+, the activation depended on the presence of phosphatidylcholine (PC) vesicles, and the activation response was different at various combinations of arachidonate and PC. Stimulation of 5LO activity was observed with Mg2+ concentrations of 0.1-1 mM, similar to the concentration range of free Mg2+ in mammalian cells. However, to observe a clear increase in 5LO hydrophobicity, a higher concentration of Mg2+ (4 mM) was required, and at this concentration also 5LO activation was optimal. Combinations of Mg2+ with ATP (containing free Mg2+ and MgATP2- complex) gave better activation of 5LO than either agent alone. This effect of Mg2+ (and ATP) could be of interest in relation to basal 5LO activity in cells not subjected to a particular stimulus." @default.
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- W2023772106 date "2000-01-29" @default.
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- W2023772106 title "Mg2+ Activates 5-Lipoxygenase in Vitro: Dependency on Concentrations of Phosphatidylcholine and Arachidonic Acid" @default.
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- W2023772106 doi "https://doi.org/10.1021/bi9919246" @default.
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